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"method": "Energy-dependent accumulation of ethylrhodamine ; Electron micrographs of ultrathin sections ",
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"animal_model": "PE cells",
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"result": true
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"rec_id": "RC03908",
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"targ": null,
"compound": "D1422: Diazepam",
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"dose": "70 μg/ml",
"time": "",
"method": "polarographic cell (1.2 ml)",
"species": "",
"animal_model": "pig kidney embryo cells",
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"result": true
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"rec_id": "RC03909",
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"targ": null,
"compound": "D1422: Diazepam",
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"dose": "150μg/ml",
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"method": "luciferin-luciferase method ",
"species": "",
"animal_model": "PE cells",
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"result": true
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"rec_id": "RC03910",
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"method": "potentiometric method ",
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"result": true
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"time": "",
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"species": "",
"animal_model": "",
"criteria": "",
"result": true
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"compound": "D1423: Leflunomide",
"paper": "295",
"func_act": "induce",
"targ_act": "inhibit",
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"time": "48 hr",
"method": "Mfn2 and Mfn1 mRNA levels; MFN2, MFN1, and porin protein levels; images of mitochondrial morphology ;",
"species": "",
"animal_model": "HeLa cells, C2C12 muscle cells, MEF cells",
"criteria": "",
"result": true
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{
"rec_id": "RC03913",
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"targ": "T004",
"compound": "D1423: Leflunomide",
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"targ_act": "inhibit",
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"time": "48 hr",
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"animal_model": "MEF cells",
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"result": true
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{
"rec_id": "RC03914",
"func": "F030501",
"targ": "T251",
"compound": "D1424: brequinar sodium",
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"time": "48hr",
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"species": "",
"animal_model": "C2C12 cells",
"criteria": "",
"result": true
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{
"rec_id": "RC03915",
"func": "F030803",
"targ": "T363",
"compound": "D1425: BGP-15",
"paper": "297",
"func_act": "induce",
"targ_act": "activate",
"dose": "50 μM",
"time": "4hr",
"method": "Polyethylene glycol (PEG) fusion assay; OPA1 GTPase assay and OPA1-OPA1 interactions",
"species": "",
"animal_model": "WRL-68 cells",
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"result": true
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{
"rec_id": "RC03916",
"func": "F030603",
"targ": null,
"compound": "D1425: BGP-15",
"paper": "297",
"func_act": "reduce",
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"dose": "50 μM",
"time": "4hr",
"method": "mERFP-labeled WRL-68 cells",
"species": "",
"animal_model": "WRL-68 cells",
"criteria": "",
"result": true
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{
"rec_id": "RC03917",
"func": "F030603",
"targ": null,
"compound": "D0183: H2O2",
"paper": "297",
"func_act": "induce",
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"method": "detected by either MitoTracker Red staining, or by mERFP transfection",
"species": "C2C12 mouse",
"animal_model": "muscle myoblasts",
"criteria": "",
"result": true
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{
"rec_id": "RC03918",
"func": null,
"targ": "T002",
"compound": "D0225: Peroxynitrite",
"paper": "298",
"func_act": "",
"targ_act": "inhibit",
"dose": "2mM, IC50=0.9mM",
"time": "",
"method": "spectrophotometrically with DCPIP at 600nm",
"species": "rat",
"animal_model": "heart mitochondria",
"criteria": "IC50",
"result": true
},
{
"rec_id": "RC03919",
"func": null,
"targ": "T001",
"compound": "D0225: Peroxynitrite",
"paper": "298",
"func_act": "",
"targ_act": "inhibit",
"dose": "2mM, IC50=1.52mM",
"time": "",
"method": "rate of NADH-dependent ferrycynide reduction at 420nm",
"species": "rat",
"animal_model": "heart mitochondria",
"criteria": "IC50",
"result": true
},
{
"rec_id": "RC03920",
"func": null,
"targ": "T005",
"compound": "D0225: Peroxynitrite",
"paper": "298",
"func_act": "",
"targ_act": "inhibit",
"dose": "2mM",
"time": "",
"method": "oxidation of reduced cytochrome c oxidase at 550nm",
"species": "rat",
"animal_model": "heart mitochondria",
"criteria": "",
"result": false
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{
"rec_id": "RC03921",
"func": null,
"targ": "T019",
"compound": "D0225: Peroxynitrite",
"paper": "298",
"func_act": "",
"targ_act": "inhibit",
"dose": "2mM, IC50=0.63mM",
"time": "",
"method": "ATPase activity was assayed by coupling to the reactions of pyruvate kinase with lactace dehydrogenase following NADH oxidation at 340nm",
"species": "rat",
"animal_model": "heart mitochondria",
"criteria": "IC50",
"result": true
},
{
"rec_id": "RC03922",
"func": "F0504",
"targ": null,
"compound": "D0029: Cisplatin",
"paper": "299",
"func_act": "increase",
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"dose": "84.96 ± 3, 158.9 ± 8.2, 372.7 ± 17.5 and 499.5 ± 15.1 μM ",
"time": "24 hr",
"method": "Intracellular ROS (a) and mitochondrial superoxide were measured by fluorescence intensity of H2DCFDA and MitoSOX respectively. ",
"species": "",
"animal_model": "Ishikawa, MDA-MB-231, PC-3 and Caco-2",
"criteria": "IC50",
"result": true
},
{
"rec_id": "RC03923",
"func": "F0504",
"targ": null,
"compound": "D1256: Dequalinium Chloride",
"paper": "299",
"func_act": "increase",
"targ_act": "",
"dose": "14.24 ± 0.59, 57.85 ± 3.59, 93.31 ± 3.21 and 179.2 ± 5.2 μM",
"time": "24 hr",
"method": "Intracellular ROS (a) and mitochondrial superoxide were measured by fluorescence intensity of H2DCFDA and MitoSOX respectively. ",
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"animal_model": "Ishikawa, MDA-MB-231, PC-3 and Caco-2",
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"result": true
},
{
"rec_id": "RC03924",
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"targ": null,
"compound": "D1256: Dequalinium Chloride",
"paper": "299",
"func_act": "decrease",
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"dose": "",
"time": "24 hr",
"method": " Relative quantification of mtDNA copy number was measured using the QuantiTect SYBR Green PCR kit",
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"animal_model": " Caco-2 and Ishikawa cells",
"criteria": "",
"result": true
},
{
"rec_id": "RC03925",
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"targ": null,
"compound": "D0029: Cisplatin",
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"dose": "",
"time": "24 hr",
"method": " Relative quantification of mtDNA copy number was measured using the QuantiTect SYBR Green PCR kit",
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"criteria": "",
"result": true
},
{
"rec_id": "RC03926",
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"compound": "D1256: Dequalinium Chloride",
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"func_act": "decrease",
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"dose": "",
"time": "1 hr",
"method": "JC-10 fluorescent dye ",
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"animal_model": "Ishikawa and Caco-2",
"criteria": "",
"result": true
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{
"rec_id": "RC03927",
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"targ": null,
"compound": "D0029: Cisplatin",
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"time": "1 or 3 hr",
"method": "JC-10 fluorescent dye ",
"species": "",
"animal_model": "Ishikawa and Caco-2",
"criteria": "",
"result": true
},
{
"rec_id": "RC03928",
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"targ": null,
"compound": "D1256: Dequalinium Chloride",
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"func_act": "induce",
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"dose": "",
"time": "24 hr",
"method": "WB was performed to measure the BCL-XL and released cytochrome c proteins from mitochondria; Caspase-3/7 activity was measured using the Caspase-Glo 3/7 reagent from the ApoTox-Glo Triplex Assay kit ",
"species": "",
"animal_model": "Ishikawa and Caco-2",
"criteria": "",
"result": true
},
{
"rec_id": "RC03929",
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"targ": null,
"compound": "D0029: Cisplatin",
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"func_act": "induce",
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"dose": "",
"time": "24 hr",
"method": "WB was performed to measure the BCL-XL and released cytochrome c proteins from mitochondria; Caspase-3/7 activity was measured using the Caspase-Glo 3/7 reagent from the ApoTox-Glo Triplex Assay kit ",
"species": "",
"animal_model": "Ishikawa and Caco-2",
"criteria": "",
"result": true
},
{
"rec_id": "RC03930",
"func": "F01",
"targ": null,
"compound": "D0152: Cadmium",
"paper": "300",
"func_act": "decrease",
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"dose": "50 μM",
"time": "3-24hr",
"method": " flow cytometry after staining the cells with the lipophilic cationic probe TMRM",
"species": "rat",
"animal_model": "PC12 cells (neuron-like )",
"criteria": "",
"result": true
},
{
"rec_id": "RC03931",
"func": "F0701",
"targ": null,
"compound": "D0152: Cadmium",
"paper": "300",
"func_act": "induce",
"targ_act": "",
"dose": "100, 500 μM",
"time": "30 min",
"method": "measured spectrofluorometrically using the fluorescent dye dichlorodihydrofluorescein diacetate (DCFH2-DA) as the ROS-sensitive probe",
"species": "rat",
"animal_model": "PC12 cells",
"criteria": "",
"result": true
},
{
"rec_id": "RC03932",
"func": "F0201",
"targ": null,
"compound": "D0152: Cadmium",
"paper": "300",
"func_act": "decrease",
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"dose": "50-500 μM",
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"method": "Cell respiration was determined polarographically with the help of Clark oxygen electrode",
"species": "rat",
"animal_model": "PC12 cells",
"criteria": "",
"result": true
},
{
"rec_id": "RC03933",
"func": "F01",
"targ": null,
"compound": "D0195: Mercury",
"paper": "300",
"func_act": "decrease",
"targ_act": "",
"dose": "50 μM",
"time": "",
"method": " flow cytometry after staining the cells with the lipophilic cationic probe TMRM",
"species": "rat",
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"criteria": "",
"result": true
},
{
"rec_id": "RC03934",
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"targ": null,
"compound": "D0195: Mercury",
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"func_act": "induce",
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"dose": "10-500 μM",
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"species": "rat",
"animal_model": "PC12 cells",
"criteria": "",
"result": true
},
{
"rec_id": "RC03935",
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"targ": null,
"compound": "D0195: Mercury",
"paper": "300",
"func_act": "decrease",
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"dose": "10-50 μM",
"time": "3hr",
"method": "Cell respiration was determined polarographically with the help of Clark oxygen electrode",
"species": "rat",
"animal_model": "PC12 cells",
"criteria": "",
"result": true
},
{
"rec_id": "RC03936",
"func": "F01",
"targ": null,
"compound": "D0032: Copper",
"paper": "300",
"func_act": "decrease",
"targ_act": "",
"dose": "50 μM",
"time": "24hr",
"method": " flow cytometry after staining the cells with the lipophilic cationic probe TMRM",
"species": "rat",
"animal_model": "PC12 cells (neuron-like )",
"criteria": "",
"result": false
},
{
"rec_id": "RC03937",
"func": "F0701",
"targ": null,
"compound": "D0032: Copper",
"paper": "300",
"func_act": "induce",
"targ_act": "",
"dose": "10-500 μM",
"time": "30min",
"method": "measured spectrofluorometrically using the fluorescent dye dichlorodihydrofluorescein diacetate (DCFH2-DA) as the ROS-sensitive probe",
"species": "rat",
"animal_model": "PC12 cells",
"criteria": "",
"result": false
},
{
"rec_id": "RC03938",
"func": "F0701",
"targ": null,
"compound": "D0032: Copper",
"paper": "300",
"func_act": "induce",
"targ_act": "",
"dose": "10-500 μM",
"time": "3hr",
"method": "measured spectrofluorometrically using the fluorescent dye dichlorodihydrofluorescein diacetate (DCFH2-DA) as the ROS-sensitive probe",
"species": "rat",
"animal_model": "PC12 cells",
"criteria": "",
"result": true
},
{
"rec_id": "RC03939",
"func": "F0701",
"targ": null,
"compound": "D0032: Copper",
"paper": "300",
"func_act": "induce",
"targ_act": "",
"dose": "500 μM",
"time": "24 hr",
"method": "measured spectrofluorometrically using the fluorescent dye dichlorodihydrofluorescein diacetate (DCFH2-DA) as the ROS-sensitive probe",
"species": "rat",
"animal_model": "PC12 cells",
"criteria": "",
"result": true
},
{
"rec_id": "RC03940",
"func": "F0201",
"targ": null,
"compound": "D0032: Copper",
"paper": "300",
"func_act": "decrease",
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"dose": "50 μM",
"time": "",
"method": "Cell respiration was determined polarographically with the help of Clark oxygen electrode",
"species": "rat",
"animal_model": "PC12 cells",
"criteria": "",
"result": true
},
{
"rec_id": "RC03941",
"func": "F02110301",
"targ": "T432",
"compound": "D1432: MCU-i4",
"paper": "301",
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"targ_act": "inhibit",
"dose": "5 μM",
"time": "",
"method": "Highigh-throughput screening assay based on aequorin. ",
"species": "",
"animal_model": " HeLa cells transfected with mitochondria-targeted aequorin (mtAeq) after cellular stimulation with histamine",
"criteria": "",
"result": true
},
{
"rec_id": "RC03942",
"func": "F02110301",
"targ": "T432",
"compound": "D1433: MCU-i11",
"paper": "301",
"func_act": "decrease",
"targ_act": "inhibit",
"dose": "5 μM",
"time": "",
"method": "Highigh-throughput screening assay based on aequorin. ",
"species": "",
"animal_model": " HeLa cells transfected with mitochondria-targeted aequorin (mtAeq) after cellular stimulation with histamine",
"criteria": "",
"result": true
},
{
"rec_id": "RC03943",
"func": "F02110301",
"targ": null,
"compound": "D1432: MCU-i4",
"paper": "301",
"func_act": "decrease",
"targ_act": "",
"dose": "10 μM",
"time": "",
"method": "Small-Scale Validation Assay (cytoplasmic- or mitochondria-targeted aequorin (cytAeq and mtAeq, respectively)",
"species": "",
"animal_model": "mouse embryonic fibroblasts (MEFs), MDA-MB-231, and HEK293T cell lines ",
"criteria": "",
"result": true
},
{
"rec_id": "RC03944",
"func": "F02110301",
"targ": null,
"compound": "D1433: MCU-i11",
"paper": "301",
"func_act": "decrease",
"targ_act": "",
"dose": "10 μM",
"time": "",
"method": "Small-Scale Validation Assay (cytoplasmic- or mitochondria-targeted aequorin (cytAeq and mtAeq, respectively)",
"species": "",
"animal_model": "mouse embryonic fibroblasts (MEFs), MDA-MB-231, and HEK293T cell lines ",
"criteria": "",
"result": true
},
{
"rec_id": "RC03945",
"func": "F01",
"targ": null,
"compound": "D1432: MCU-i4",
"paper": "301",
"func_act": "decrease",
"targ_act": "",
"dose": "10 μM",
"time": "15min",
"method": "TMRM (using the Mitochondrial Membrane Potential Indicator Kit)",
"species": "",
"animal_model": "HeLa cells",
"criteria": "",
"result": false
},
{
"rec_id": "RC03946",
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"targ": null,
"compound": "D1433: MCU-i11",
"paper": "301",
"func_act": "decrease",
"targ_act": "",
"dose": "10 μM",
"time": "15min",
"method": "TMRM (using the Mitochondrial Membrane Potential Indicator Kit)",
"species": "",
"animal_model": "HeLa cells",
"criteria": "",
"result": false
},
{
"rec_id": "RC03947",
"func": "F02110301",
"targ": "T432",
"compound": "D1432: MCU-i4",
"paper": "301",
"func_act": "decrease",
"targ_act": "inhibit",
"dose": "8.4 μM(Micu1), 18 μM (Micu1-Micu2)",
"time": "",
"method": "Purified recombinant Micu1, Micu2, and the dimeric Micu1-Micu2 proteins were immobilized on different channels of a SPR sensor chip surface.",
"species": "",
"animal_model": "Purified recombinant Micu1, Micu2",
"criteria": "IC50",
"result": true
},
{
"rec_id": "RC03948",
"func": "F02110301",
"targ": "T432",
"compound": "D1433: MCU-i11",
"paper": "301",
"func_act": "decrease",
"targ_act": "inhibit",
"dose": "2.9 μM(Micu1), 2.7 μM (Micu1-Micu2)",
"time": "",
"method": "Purified recombinant Micu1, Micu2, and the dimeric Micu1-Micu2 proteins were immobilized on different channels of a SPR sensor chip surface.",
"species": "",
"animal_model": "Purified recombinant Micu1, Micu2",
"criteria": "IC50",
"result": true
},
{
"rec_id": "RC03949",
"func": "F02110301",
"targ": null,
"compound": "D1432: MCU-i4",
"paper": "301",
"func_act": "decrease",
"targ_act": "",
"dose": "10 μM",
"time": "",
"method": "measured mitochondrial Ca2+ uptake in flexor digitorum brevis (FDB) myofibers freshly isolated from adult mice and previously transfected in vivo with the mitochondria-targeted Ca2+ probe 4mt-GCaMP6f ",
"species": "",
"animal_model": "Skeletal Muscle Fibers",
"criteria": "",
"result": true
},
{
"rec_id": "RC03950",
"func": "F02110301",
"targ": null,
"compound": "D1433: MCU-i11",
"paper": "301",
"func_act": "decrease",
"targ_act": "",
"dose": "10 μM",
"time": "",
"method": "measured mitochondrial Ca2+ uptake in flexor digitorum brevis (FDB) myofibers freshly isolated from adult mice and previously transfected in vivo with the mitochondria-targeted Ca2+ probe 4mt-GCaMP6f ",
"species": "",
"animal_model": "Skeletal Muscle Fibers",
"criteria": "",
"result": true
},
{
"rec_id": "RC03951",
"func": "F02110301",
"targ": "T205",
"compound": "D1435: DS16570511",
"paper": "302",
"func_act": "decrease",
"targ_act": "inhibit",
"dose": "7 μM",
"time": "",
"method": "HEK293A cell lines expressing the mitochondria-targeted Ca2+ indicator protein aequorin ",
"species": "",
"animal_model": "HEK293A cells ",
"criteria": "IC50",
"result": true
},
{
"rec_id": "RC03952",
"func": "F030804",
"targ": "T358",
"compound": "D0277: Mdivi-1",
"paper": "296",
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"targ_act": "inhibitor",
"dose": "",
"time": "",
"method": "",
"species": "",
"animal_model": "",
"criteria": "",
"result": true
},
{
"rec_id": "RC03954",
"func": null,
"targ": "T002",
"compound": "D0404: Thenoyltrifluoroacetone",
"paper": "303",
"func_act": "",
"targ_act": "inhibitor",
"dose": "25 μM",
"time": "",
"method": "The reduction of ferricyanide by succinate cata-lyzed by SDH was monitored spectrophotometrically",
"species": "rat",
"animal_model": "liver mitochondria",
"criteria": "",
"result": true
},
{
"rec_id": "RC03955",
"func": null,
"targ": "T002",
"compound": "D0073: Ketoconazole",
"paper": "303",
"func_act": "",
"targ_act": "inhibitor",
"dose": "40-100 μM",
"time": "",
"method": "The reduction of ferricyanide by succinate cata-lyzed by SDH was monitored spectrophotometrically",
"species": "rat",
"animal_model": "liver mitochondria",
"criteria": "",
"result": true
},
{
"rec_id": "RC03956",
"func": "F02010103",
"targ": null,
"compound": "D0073: Ketoconazole",
"paper": "303",
"func_act": "inhibit",
"targ_act": "",
"dose": "I50=32, 74, 65, 75, 500μM for substrates Glutamate/Malate, Pyruvate/Malate, Ornithine/Malate, Arginine/Malate and Succinate repectively",
"time": "",
"method": "Clark electrode",
"species": "rat",
"animal_model": "liver mitochondria",
"criteria": "",
"result": true
},
{
"rec_id": "RC03957",
"func": "F02010103",
"targ": null,
"compound": "D0417: Fluconazole",
"paper": "303",
"func_act": "inhibit",
"targ_act": "",
"dose": "",
"time": "",
"method": "Clark electrode",
"species": "rat",
"animal_model": "liver mitochondria",
"criteria": "",
"result": false
},
{
"rec_id": "RC03958",
"func": "F02010103",
"targ": null,
"compound": "D1437: Miconazole",
"paper": "303",
"func_act": "inhibit",
"targ_act": "",
"dose": "I50=100, 160, 160, 170, 150 μM for substrates Glutamate/Malate, Pyruvate/Malate, Ornithine/Malate, Arginine/Malate and Succinate repectively",
"time": "",
"method": "Clark electrode",
"species": "rat",
"animal_model": "liver mitochondria",
"criteria": "",
"result": true
},
{
"rec_id": "RC03959",
"func": "F020603",
"targ": null,
"compound": "D1426: amphotericin B",
"paper": "304",
"func_act": "induce",
"targ_act": "",
"dose": "2.5mg/ml",
"time": "",
"method": "the formation of MDAs by antifungal drug-derived ROS in A. fumigatus was determined using the thiobarbituric acid (TBA) assay.",
"species": "Aspergillus fumigatus",
"animal_model": "",
"criteria": "",
"result": true
},
{
"rec_id": "RC03960",
"func": "F020603",
"targ": null,
"compound": "D1427: caspofungin",
"paper": "304",
"func_act": "induce",
"targ_act": "",
"dose": "",
"time": "",
"method": "lipid peroxidation using the thiobarbituric acid assay: the formation of malondialdehydes (MDAs) by antifungal drug-derived ROS in A. fumigatus was determined using the thiobarbituric acid (TBA) assay.",
"species": "Aspergillus fumigatus",
"animal_model": "",
"criteria": "",
"result": false
},
{
"rec_id": "RC03961",
"func": "F020603",
"targ": null,
"compound": "D1399: Terbinafine",
"paper": "304",
"func_act": "induce",
"targ_act": "",
"dose": "4mg/ml",
"time": "",
"method": "lipid peroxidation using the thiobarbituric acid assay",
"species": "Aspergillus fumigatus",
"animal_model": "",
"criteria": "",
"result": true
},
{
"rec_id": "RC03962",
"func": "F020603",
"targ": null,
"compound": "D1429: itraconazole",
"paper": "304",
"func_act": "induce",
"targ_act": "",
"dose": "4mg/ml",
"time": "",
"method": "lipid peroxidation using the thiobarbituric acid assay",
"species": "Aspergillus fumigatus",
"animal_model": "",
"criteria": "",
"result": true
},
{
"rec_id": "RC03963",
"func": "F020603",
"targ": null,
"compound": "D1426: amphotericin B",
"paper": "304",
"func_act": "induce",
"targ_act": "",
"dose": "2.5mg/ml",
"time": "",
"method": "mitochondrion-specific lipid peroxidation probe MitoPerOx",
"species": "Aspergillus fumigatus",
"animal_model": "",
"criteria": "",
"result": true
},
{
"rec_id": "RC03964",
"func": "F020603",
"targ": null,
"compound": "D1427: caspofungin",
"paper": "304",
"func_act": "induce",
"targ_act": "",
"dose": "",
"time": "",
"method": "mitochondrion-specific lipid peroxidation probe MitoPerOx",
"species": "Aspergillus fumigatus",
"animal_model": "",
"criteria": "",
"result": false
},
{
"rec_id": "RC03965",
"func": "F020603",
"targ": null,
"compound": "D1399: Terbinafine",
"paper": "304",
"func_act": "induce",
"targ_act": "",
"dose": "4mg/ml",
"time": "3min, 5min, 15min",
"method": "mitochondrion-specific lipid peroxidation probe MitoPerOx",
"species": "Aspergillus fumigatus",
"animal_model": "",
"criteria": "",
"result": true
},
{
"rec_id": "RC03966",
"func": "F020603",
"targ": null,
"compound": "D1429: itraconazole",
"paper": "304",
"func_act": "induce",
"targ_act": "",
"dose": "4mg/ml",
"time": "",
"method": "mitochondrion-specific lipid peroxidation probe MitoPerOx",
"species": "Aspergillus fumigatus",
"animal_model": "",
"criteria": "",
"result": true
},
{
"rec_id": "RC03967",
"func": "F0504",
"targ": null,
"compound": "D1426: amphotericin B",
"paper": "304",
"func_act": "induce",
"targ_act": "",
"dose": "2.5mg/ml",
"time": "",
"method": "oxidant-sensing fluorescent probe 2′,7′-dichlorofluorescin diacetate t",
"species": "Aspergillus fumigatus",
"animal_model": "",
"criteria": "",
"result": true
},
{
"rec_id": "RC03968",
"func": "F0504",
"targ": null,
"compound": "D1427: caspofungin",
"paper": "304",
"func_act": "induce",
"targ_act": "",
"dose": "0.5mg/ml",
"time": "",
"method": "oxidant-sensing fluorescent probe 2′,7′-dichlorofluorescin diacetate t",
"species": "Aspergillus fumigatus",
"animal_model": "",
"criteria": "",
"result": false
},
{
"rec_id": "RC03969",
"func": "F0504",
"targ": null,
"compound": "D1399: Terbinafine",
"paper": "304",
"func_act": "induce",
"targ_act": "",
"dose": "4mg/ml",
"time": "",
"method": "oxidant-sensing fluorescent probe 2′,7′-dichlorofluorescin diacetate t",
"species": "Aspergillus fumigatus",
"animal_model": "",
"criteria": "",
"result": true
},
{
"rec_id": "RC03970",
"func": "F0504",
"targ": null,
"compound": "D1429: itraconazole",
"paper": "304",
"func_act": "induce",
"targ_act": "",
"dose": "4mg/ml",
"time": "",
"method": "oxidant-sensing fluorescent probe 2′,7′-dichlorofluorescin diacetate t",
"species": "Aspergillus fumigatus",
"animal_model": "",
"criteria": "",
"result": true
},
{
"rec_id": "RC03971",
"func": "F01",
"targ": null,
"compound": "D0900: dodecyl gallate",
"paper": "305",
"func_act": "decrease",
"targ_act": "",
"dose": "2.13 ± 0.2 μM",
"time": "72hr",
"method": "measured by flow cytometry using JC-1 dye (BD MitoScreen kit)",
"species": "Epimastigote forms of Trypanosoma cruzi of Y strain,",
"animal_model": "",
"criteria": "IC50",
"result": true
},
{
"rec_id": "RC03972",
"func": "F01",
"targ": null,
"compound": "D1070: propyl gallate",
"paper": "305",
"func_act": "decrease",
"targ_act": "",
"dose": ">100 μM",
"time": "72hr",
"method": "measured by flow cytometry using JC-1 dye (BD MitoScreen kit)",
"species": "Epimastigote forms of Trypanosoma cruzi of Y strain,",
"animal_model": "",
"criteria": "IC50",
"result": false
},
{
"rec_id": "RC03973",
"func": "F01",
"targ": null,
"compound": "D1441: Benznidazole",
"paper": "305",
"func_act": "decrease",
"targ_act": "",
"dose": "34μM",
"time": "",
"method": "measured by flow cytometry using JC-1 dye (BD MitoScreen kit)",
"species": "Epimastigote forms of Trypanosoma cruzi of Y strain,",
"animal_model": "",
"criteria": "IC50",
"result": true
},
{
"rec_id": "RC03974",
"func": "F030802",
"targ": "T419",
"compound": "D1430: XCT790",
"paper": "308",
"func_act": "inhibit",
"targ_act": "inhibitor",
"dose": "",
"time": "",
"method": "",
"species": "",
"animal_model": "",
"criteria": "",
"result": true
},
{
"rec_id": "RC04664",
"func": "F0504",
"targ": "T387",
"compound": "D0005: Amiodarone",
"paper": "306",
"func_act": "Increase",
"targ_act": "increase",
"dose": "10 µM",
"time": "1 hour",
"method": "High-content screening assay",
"species": "Human",
"animal_model": "HepG2",
"criteria": "MEC",
"result": true
},
{
"rec_id": "RC03976",
"func": null,
"targ": "T004",
"compound": "D1444: decylubiquinol",
"paper": "309",
"func_act": "",
"targ_act": "binding",
"dose": "19.4 ± 7.17 (female), 25.00 ± 10.30 (male)",
"time": "",
"method": "isothermal titration calorimetry (ITC)",
"species": "zebrafish (adult, 24 wk)",
"animal_model": " isolate mitochondria from liver",
"criteria": "Kd ",
"result": true
},
{
"rec_id": "RC03977",
"func": null,
"targ": "T004",
"compound": "D1445: hexachlorobenzene",
"paper": "309",
"func_act": "",
"targ_act": "",
"dose": "",
"time": "",
"method": "complex III activity assay: decylubiquinol-dependent reduction of cytochrome c by mitochondrial complex III.",
"species": "zebrafish (adult, 24 wk)",
"animal_model": "",
"criteria": "",
"result": false
},
{
"rec_id": "RC03978",
"func": "F030603",
"targ": "T358",
"compound": "D1443: P110",
"paper": "310",
"func_act": "inhibit",
"targ_act": "inhibits",
"dose": "1 µM",
"time": "30min",
"method": "microscopy images",
"species": "",
"animal_model": "Cultured SH-SY5Y cells incubated with MPP+ (2 mM, for 4 hours) or CCCP (5 µM, for 30 minutes). The cells were then stained with anti-Tom20 antibody (green) and Hoechst stain.",
"criteria": "",
"result": true
},
{
"rec_id": "RC03979",
"func": "F0504",
"targ": "T358",
"compound": "D1443: P110",
"paper": "310",
"func_act": "reduce",
"targ_act": "",
"dose": "1 µM",
"time": "2hr",
"method": "Mitochondrial superoxide production was determined using the mitochondrial superoxide indicator MitoSOX Red. Total ROS production was determined in SH-SY5Y cells using CM2-HDCFA.",
"species": "",
"animal_model": "SH-SY5Y cells were treated with P110 (1 µM) followed by incubation of MPP+ (2 mM) for 2 hours.",
"criteria": "",
"result": true
},
{
"rec_id": "RC03980",
"func": "F01",
"targ": null,
"compound": "D1443: P110",
"paper": "310",
"func_act": "enhance",
"targ_act": "",
"dose": "1 µM",
"time": "",
"method": "TMRM",
"species": "mouse",
"animal_model": "isolated liver mitochondria",
"criteria": "",
"result": true
},
{
"rec_id": "RC03981",
"func": "F02110302",
"targ": null,
"compound": "D1449: neomycin",
"paper": "315",
"func_act": "increase",
"targ_act": "",
"dose": " 400 μM",
"time": "",
"method": "measrueing cytoplasmic oxidation (HyPer) and mitochondrial Ca2+ uptake (mitoRGECO)",
"species": "zebrafish",
"animal_model": "zebrafish lateral line hair",
"criteria": "",
"result": true
},
{
"rec_id": "RC03982",
"func": "F0504",
"targ": null,
"compound": "D1449: neomycin",
"paper": "315",
"func_act": "increase",
"targ_act": "",
"dose": "50 μM",
"time": "",
"method": "use ROS indicator dye cellROX green ( it labels a number of intracellular compartments, including cytoplasm, nucleus, and mitochondria)",
"species": "zebrafish",
"animal_model": "zebrafish lateral line hair",
"criteria": "",
"result": true
},
{
"rec_id": "RC03983",
"func": "F021214",
"targ": null,
"compound": "D1411: Gentamicin",
"paper": "312",
"func_act": "decrease",
"targ_act": "",
"dose": "300 μg/ml",
"time": "1hr",
"method": "scanning confocal microscope (NADH autofluorescence); T1 imaging buffer contained 5 mM glucose. T2 imaging buffer contained 10 mM glucose, 3 mM glutamate and 2 mM pyruvate.",
"species": " postnatal day 6 (P6±1d) FVB mice",
"animal_model": "apical turn outer hair cells ( low-frequency processing OHCs) from intact cochlear (organ of Corti) explants",
"criteria": "",
"result": false
},
{
"rec_id": "RC03984",
"func": "F021214",
"targ": null,
"compound": "D1411: Gentamicin",
"paper": "312",
"func_act": "decrease",
"targ_act": "",
"dose": "300 μg/ml",
"time": "1hr",
"method": "scanning confocal microscope (NADH autofluorescence); T1 imaging buffer contained 5 mM glucose. T2 imaging buffer contained 10 mM glucose, 3 mM glutamate and 2 mM pyruvate.",
"species": " postnatal day 6 (P6±1d) FVB mice",
"animal_model": "apical turn inner hair cells ( low-frequency processing IHCs) from intact cochlear (organ of Corti) explants",
"criteria": "",
"result": false
},
{
"rec_id": "RC03985",
"func": "F021214",
"targ": null,
"compound": "D1411: Gentamicin",
"paper": "312",
"func_act": "decrease",
"targ_act": "",
"dose": "300 μg/ml",
"time": "1hr",
"method": "scanning confocal microscope (NADH autofluorescence); T1 imaging buffer contained 5 mM glucose. T2 imaging buffer contained 10 mM glucose, 3 mM glutamate and 2 mM pyruvate.",
"species": " postnatal day 6 (P6±1d) FVB mice",
"animal_model": "basal turn outer hair cells ( low-frequency processing OHCs) from intact cochlear (organ of Corti) explants",
"criteria": "",
"result": true
},
{
"rec_id": "RC03986",
"func": "F021214",
"targ": null,
"compound": "D1411: Gentamicin",
"paper": "312",
"func_act": "decrease",
"targ_act": "",
"dose": "300 μg/ml",
"time": "1hr",
"method": "scanning confocal microscope (autofluorescence); T1 imaging buffer contained 5 mM glucose. T2 imaging buffer contained 10 mM glucose, 3 mM glutamate and 2 mM pyruvate.",
"species": " postnatal day 6 (P6±1d) FVB mice",
"animal_model": "basal turn inner hair cells ( high-frequency processing IHCs) from intact cochlear (organ of Corti) explants",
"criteria": "",
"result": false
},
{
"rec_id": "RC03987",
"func": null,
"targ": "T002",
"compound": "D1411: Gentamicin",
"paper": "312",
"func_act": "",
"targ_act": "inhibit",
"dose": "300 μg/ml",
"time": "1hr",
"method": " Succinate dehydrogenase (SDH) histochemistry (tetranitro-blue accumulation)",
"species": " postnatal day 6 (P6±1d) FVB mice",
"animal_model": "apical turn ( low-frequency processing) outer hair cells (OHCs) from intact cochlear (organ of Corti) explants",
"criteria": "",
"result": true
},
{
"rec_id": "RC03988",
"func": null,
"targ": "T002",
"compound": "D1411: Gentamicin",
"paper": "312",
"func_act": "",
"targ_act": "inhibit",
"dose": "300 μg/ml",
"time": "1hr",
"method": " Succinate dehydrogenase (SDH) histochemistry (tetranitro-blue accumulation)",
"species": " postnatal day 6 (P6±1d) FVB mice",
"animal_model": "apical turn ( low-frequency processing) outer hair cells (OHCs) from intact cochlear (organ of Corti) explants",
"criteria": "",
"result": false
},
{
"rec_id": "RC03989",
"func": null,
"targ": "T002",
"compound": "D1411: Gentamicin",
"paper": "312",
"func_act": "",
"targ_act": "inhibit",
"dose": "300 μg/ml",
"time": "1hr",
"method": " Succinate dehydrogenase (SDH) histochemistry (tetranitro-blue accumulation)",
"species": " postnatal day 6 (P6±1d) FVB mice",
"animal_model": "basal turn(high-frequency regions of the cochlea) outer hair cells from intact cochlear (organ of Corti) explants",
"criteria": "",
"result": true
},
{
"rec_id": "RC03990",
"func": null,
"targ": "T002",
"compound": "D1411: Gentamicin",
"paper": "312",
"func_act": "",
"targ_act": "inhibit",
"dose": "300 μg/ml",
"time": "1hr",
"method": " Succinate dehydrogenase (SDH) histochemistry (tetranitro-blue accumulation)",
"species": " postnatal day 6 (P6±1d) FVB mice",
"animal_model": "basal turn(high-frequency regions of the cochlea) outer hair cells from intact cochlear (organ of Corti) explants",
"criteria": "",
"result": false
},
{
"rec_id": "RC03991",
"func": "F0504",
"targ": null,
"compound": "D1404: Staurosporine",
"paper": "313",
"func_act": "increase",
"targ_act": "",
"dose": "2 µM",
"time": "24hr",
"method": "mt-roGFP and mitoSOX",
"species": "",
"animal_model": " U2OS",
"criteria": "",
"result": true
},
{
"rec_id": "RC03992",
"func": "F0504",
"targ": null,
"compound": "D1448: camptothecin",
"paper": "313",
"func_act": "increase",
"targ_act": "",
"dose": "30 µM",
"time": "24hr",
"method": "mt-roGFP and mitoSOX",
"species": "",
"animal_model": " U2OS",
"criteria": "",
"result": true
},
{
"rec_id": "RC03993",
"func": "F0504",
"targ": null,
"compound": "D0194: Menadione",
"paper": "313",
"func_act": "increase",
"targ_act": "",
"dose": "20 µM",
"time": "24hr",
"method": "mt-roGFP and mitoSOX",
"species": "",
"animal_model": " U2OS",
"criteria": "",
"result": true
},
{
"rec_id": "RC03994",
"func": "F0504",
"targ": null,
"compound": "D0029: Cisplatin",
"paper": "313",
"func_act": "increase",
"targ_act": "",
"dose": "150 µM",
"time": "24hr",
"method": "U2OS cells stably expressing mt-roGFP and HyPer-red targeted at mitochondria",
"species": "",
"animal_model": " U2OS",
"criteria": "",
"result": true
},
{
"rec_id": "RC03995",
"func": "F0504",
"targ": null,
"compound": "D1448: camptothecin",
"paper": "313",
"func_act": "increase",
"targ_act": "",
"dose": "30 µM",
"time": "24hr",
"method": "U2OS cells stably expressing mt-roGFP and HyPer-red targeted at mitochondria",
"species": "",
"animal_model": " U2OS",
"criteria": "",
"result": true
},
{
"rec_id": "RC03996",
"func": "F0104",
"targ": null,
"compound": "D0029: Cisplatin",
"paper": "313",
"func_act": "induce",
"targ_act": "",
"dose": "",
"time": "",
"method": "release of Smac m cherry(IMS-RP probe is a non-functional variant of mitochondrial protein Smac - mCherry fusion protein that acts as an indicator of mitochondrial permeabilization in live cells)",
"species": "",
"animal_model": " U2OS",
"criteria": "",
"result": true
},
{
"rec_id": "RC03997",
"func": "F0504",
"targ": null,
"compound": "D0371: Mitoquinone",
"paper": "313",
"func_act": "reduce",
"targ_act": "",
"dose": "",
"time": "",
"method": "mt-roGFP ratio change (Quantitative representation of the percentage of cell death in cells pre-treated with MitoQ followed by drug treatment or drug alone as indicated for 24 h)",
"species": "",
"animal_model": " U2OS",
"criteria": "",
"result": true
},
{
"rec_id": "RC03998",
"func": "F060301",
"targ": "T231",
"compound": "D1447: LDC203974",
"paper": "314",
"func_act": "inhibit",
"targ_act": "inhibit",
"dose": " 0–10 μM",
"time": "",
"method": "Single nucleotide incorporation assays at varying ATP concentrations",
"species": "",
"animal_model": "",
"criteria": "Vmax app, maximum reaction rate; Km app, substrate affinity",
"result": true
},
{
"rec_id": "RC03999",
"func": "F060301",
"targ": "T231",
"compound": "D1447: LDC203974",
"paper": "314",
"func_act": "inhibit",
"targ_act": "inhibit",
"dose": "743nM",
"time": "",
"method": "Microscale thermophoresis binding curves in the absence or presence of 10 μM IMT1B ",
"species": "",
"animal_model": "",
"criteria": "Kd",
"result": true
},
{
"rec_id": "RC04000",
"func": "F060301",
"targ": "T231",
"compound": "D1447: LDC203974",
"paper": "314",
"func_act": "inhibit",
"targ_act": "inhibit",
"dose": "10 μM",
"time": "6hr",
"method": " Mitochondrial transcript levels of ND1 and CYTB",
"species": "",
"animal_model": "HeLa cells",
"criteria": "",
"result": true
},
{
"rec_id": "RC04001",
"func": "F01",
"targ": null,
"compound": "D1411: Gentamicin",
"paper": "316",
"func_act": "reduce",
"targ_act": "",
"dose": " 2.5 mM",
"time": "",
"method": "MtMP was investigated with the use of safranin, a biological stain with an excitation wavelength of 495 nm and an emission wavelength of 587 nm. ",
"species": "rat",
"animal_model": "isolated rat liver (and kidney) mitochondria",
"criteria": "",
"result": true
},
{
"rec_id": "RC04002",
"func": "F01",
"targ": null,
"compound": "D1411: Gentamicin",
"paper": "316",
"func_act": "reduce",
"targ_act": "",
"dose": "",
"time": "",
"method": "Rhodamine-123",
"species": "mouse",
"animal_model": "Mouse Cochlear Culture Sensory Hair Cells",
"criteria": "",
"result": true
},
{
"rec_id": "RC04003",
"func": "F050401",
"targ": null,
"compound": "D1411: Gentamicin",
"paper": "316",
"func_act": "reduce",
"targ_act": "",
"dose": "5mM",
"time": "10min",
"method": "Mitochondrial ROS production can be measured using Amplex Red, a dye that fluoresces dependent on hydrogen peroxide (H2O2) levels. ",
"species": "rat",
"animal_model": "isolated rat liver (and kidney) mitochondria",
"criteria": "",
"result": true
},
{
"rec_id": "RC04004",
"func": "F02010102",
"targ": null,
"compound": "D1411: Gentamicin",
"paper": "316",
"func_act": "increase",
"targ_act": "",
"dose": "5mM",
"time": "10min",
"method": "Oroboros O2K Oxygraph, complex II-based state 4 respiration ",
"species": "rat",
"animal_model": "isolated rat liver (and kidney) mitochondria",
"criteria": "",
"result": true
},
{
"rec_id": "RC04005",
"func": "F02010106",
"targ": null,
"compound": "D1411: Gentamicin",
"paper": "316",
"func_act": "decrease",
"targ_act": "",
"dose": "5mM",
"time": "10min",
"method": "Oroboros O2K Oxygraph, complex II-based state 3u respiration ",
"species": "rat",
"animal_model": "isolated rat liver (and kidney) mitochondria",
"criteria": "",
"result": true
}
]
}
