D1484 | RO-25-0187
| Toxicity | Dose | Time | Species | Model | Method | Action | Positive criterion | Reference |
|---|---|---|---|---|---|---|---|---|
| MITOCHONDRIAL FATTY ACID BETA OXIDATION | 25.5/>100 | human/rat | hepatocytes | Fatty acid oxidation (FAO) was determined by measuring 14CO2 release from 14C-labeled palmitate | inhibition | IC50 (μM) | 333 | |
| SYNTHESIS OF KETONE BODY | 1.5 | Wistar rat | hepatocytes | ketone bodies (KB = β-hydroxybutyrate + acetoacetate) were determined with a commercially available kit (Autokit 3-HB from Wako) | inhibition | IC50 (μM) | 333 | |
| Target | Dose | Time | Species | Model | Method | Action | Positive criterion | Reference |
|---|---|---|---|---|---|---|---|---|
| Carnitine O-palmitoyltransferase 1, liver isoform, CPT1-L, EC 2.3.1.21 | 2.4/− | human/rat | recombinant Pichia pastoris Membrane Preparations | spectrophotometric assay with DTNB | inhibition | IC50 (μM) | 333 | |
| Carnitine O-palmitoyltransferase 1, muscle isoform | 0.16 | human/rat | recombinant Pichia pastoris Membrane Preparations | spectrophotometric assay with DTNB | inhibition | IC50 (μM) | 333 | |
| Carnitine O-palmitoyltransferase 2, mitochondrial, EC 2.3.1.21 | 4.7/>100 | human/rat | recombinant Pichia pastoris Membrane Preparations | spectrophotometric assay with DTNB | inhibition | IC50 (μM) | 333 | |