Inhibition of succinic dehydrogenase and F0F1-ATP synthase by 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS).

Authors

Bernardes, C F; Meyer-Fernandes, J R; Martins, O B; Vercesi, A E

Publication Year 1997
Journal Zeitschrift fur Naturforschung. C, Journal of Biosciences
Chapter
Pages 799-806
Volume 52
Issue 11-12
Issn
Isbn
PMID 9463937.0
PMCID
DOI 10.1515/znc-1997-11-1212
URL http://dx.doi.org/10.1515/znc-1997-11-1212

This study shows that incubation of rat liver mitochondria in the presence of the thiol/ amino reagent 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) is followed by inhibition of both succinate supported respiration and oxidative phosphorylation. Half-maximal inhibition of succinic dehydrogenase activity and succinate oxidation by mitochondria was attained at 55.3 and 60.8 microM DIDS, respectively. DIDS did inhibit the net ATP synthesis and ATP< =>[32P]Pi exchange reaction catalyzed by submitochondrial particles in a dose-dependent manner (Ki = 31.7 microM and Ki = 32.7 microM), respectively. The hydrolytic activities of uncoupled heart submitochondrial particles and purified F1-ATPase were also inhibited 50% by 31.9 and 20.9 microM DIDS, respectively.