Inhibition of Escherichia coli H+-ATPase by venturicidin, oligomycin and ossamycin.


Perlin, D S; Latchney, L R; Senior, A E

Publication Year 1985
Journal Biochimica et Biophysica Acta
Pages 238-244
Volume 807
Issue 3
PMID 2859888.0
DOI 10.1016/0005-2728(85)90254-3

The antibiotics venturicidin, oligomycin and ossamycin were investigated as potential inhibitors of the Escherichia coli H+-ATPase. It was found that venturicidin strongly inhibited ATP-driven proton transport and ATP hydrolysis, while oligomycin weakly inhibited these functions. Inhibition of the H+-ATPase by venturicidin and oligomycin was correlated with inhibition of F0-mediate proton transport. Both inhibitors were found to interfere with the covalent reaction between dicyclohexyl[14C]carbodiimide and the F0 subunit c (uncE protein). Ossamycin had no direct inhibitory effect on E. coli F0 or F1; rather, it was found to uncouple ATP hydrolysis from proton transport.