Diazepam inhibits cell respiration and induces fragmentation of mitochondrial reticulum.


Vorobjev, I A; Zorov, D B

Publication Year 1905
Journal FEBS Letters
Pages 311-314
Volume 163
Issue 2
PMID 6641946.0
DOI 10.1016/0014-5793(83)80842-4
URL http://dx.doi.org/10.1016/0014-5793(83)80842-4

Diazepam (70-150 micrograms/ml) significantly inhibits oxygen consumption by pig kidney embryo cells and causes the cellular ATP level to fall. The maximum inhibitory effect develops after 1.5-2.5 h of diazepam treatment. In isolated mitochondria diazepam inhibits respiration in state 2 and 3u with glutamate and in state 3u with succinate. Ethylrhodamine staining and electron microscopic study reveal fragmentation of mitochondria in living cells.