T118 Source: Uniprot

enzymePeptidyl-prolyl cis-trans isomerase F, mitochondrial (PPIase F)
Rotamase Isomerase
Full Name Peptidyl-prolyl cis-trans isomerase F, mitochondrial
Protein Names Peptidyl-prolyl cis-trans isomerase F, mitochondrial (PPIase F) (EC 5.2.1.8) (Cyclophilin D) (CyP-D) (CypD) (Cyclophilin F) (Mitochondrial cyclophilin) (CyP-M) (Rotamase F)
Subcellular Location Mitochondrion matrix
EC numbers 5.2.1.8
Uniprot Accession P30405
Uniprot Name PPIF_HUMAN
Gene symbol PPIF
Gene Name Peptidylprolyl isomerase F
Ensembl ENSG00000108179

Compound Dose Time Species Model Method Result Positive criterion Reference
Cyclosporin A 5 μM mouse embryonic fibroblasts (MEFs) and SH-SY5Y cells Mitochondrial population levels were determined by flow cytometry using MitoTracker Deep Red (MTDR) dye Positive 252
JW47 504.5 ± 9.2 nM (CypA), 298.0 ± 17.9 nM( K133I CypD) Fluorescence Polarization Assay to measure affinity of ligands to cyclophilins (fluorescein-PEG-CsA ligand as the probe) Positive Ki 324
Cyclosporin A 22.5 ± 4.3nM (CypA), 1.4 ± 1.6 nM( K133I CypD) Fluorescence Polarization Assay to measure affinity of ligands to cyclophilins (fluorescein-PEG-CsA ligand as the probe) Positive Ki 324