D0199 | Methylene Blue
V
V04CG05 Methylthioninium chloride
[V04CG] Tests for gastric secretion
[V04C] OTHER DIAGNOSTIC AGENTS
[V04] DIAGNOSTIC AGENTS
[V] Various ATC structures
V03AB17 Methylthioninium chloride
[V03AB] Antidotes
[V03A] ALL OTHER THERAPEUTIC PRODUCTS
[V03] ALL OTHER THERAPEUTIC PRODUCTS
[V] Various ATC structures
| Toxicity | Dose | Time | Species | Model | Method | Action | Positive criterion | Reference |
|---|---|---|---|---|---|---|---|---|
| MEMBRANE POTENTIAL | 2.88±1.33 | human | qHTS-HepG2 | MMP assay | decrease | IC50 | 163 | |
| MEMBRANE POTENTIAL | 2.75 | human | HepG2 | MMP assay | decrease | IC50 | 163 | |
| MEMBRANE POTENTIAL | rat | hepatocytes | MMP assay | Negative | IC50 | 163 | ||
| Pictogram | Signal | Statements | Precautionary Statement Codes |
|---|---|---|---|
  |
Danger |
Aggregated GHS information provided by 262 companies from 13 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies. Reported as not meeting GHS hazard criteria by 19 of 262 companies. For more detailed information, please visit ECHA C&L website Of the 11 notification(s) provided by 243 of 262 companies with hazard statement code(s): H302 (99.59%): Harmful if swallowed [Warning Acute toxicity, oral] H318 (27.98%): Causes serious eye damage [Danger Serious eye damage/eye irritation] Information may vary between notifications depending on impurities, additives, and other factors. The percentage value in parenthesis indicates the notified classification ratio from companies that provide hazard codes. Only hazard codes with percentage values above 10% are shown. |
P264, P270, P280, P301+P312, P305+P351+P338, P310, P330, and P501; (The corresponding statement to each P-code can be found at the GHS Classification page.) |
|
|
Danger |
Aggregated GHS information provided by 2 companies from 1 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies. H302 (100%): Harmful if swallowed [Warning Acute toxicity, oral] H318 (100%): Causes serious eye damage [Danger Serious eye damage/eye irritation] Information may vary between notifications depending on impurities, additives, and other factors. The percentage value in parenthesis indicates the notified classification ratio from companies that provide hazard codes. Only hazard codes with percentage values above 10% are shown. |
P264, P270, P280, P301+P312, P305+P351+P338, P310, P330, and P501; (The corresponding statement to each P-code can be found at the GHS Classification page.) |
 |
Danger |
H302: Harmful if swallowed [Warning Acute toxicity, oral] H361: Suspected of damaging fertility or the unborn child [Warning Reproductive toxicity] H370: Causes damage to organs [Danger Specific target organ toxicity, single exposure] H372: Causes damage to organs through prolonged or repeated exposure [Danger Specific target organ toxicity, repeated exposure] H402: Harmful to aquatic life [Hazardous to the aquatic environment, acute hazard] H412: Harmful to aquatic life with long lasting effects [Hazardous to the aquatic environment, long-term hazard] |
P201, P202, P260, P264, P270, P273, P281, P301+P312, P307+P311, P308+P313, P314, P321, P330, P405, and P501; (The corresponding statement to each P-code can be found at the GHS Classification page.) |
| Organism | Test type | Route | Dose (normalized dose) | Effect | Source |
|---|---|---|---|---|---|
| guinea pig | LDLo | subcutaneous | 300mg/kg (300mg/kg) | "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." Vol. 4, Pg. 1366, 1935. | |
| dog | LDLo | intravenous | 50mg/kg (50mg/kg) | "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." Vol. 4, Pg. 1366, 1935. | |
| domestic animals - goat/sheep | LD50 | intravenous | 42300ug/kg (42.3mg/kg) | Journal of Veterinary Pharmacology and Therapeutics. Vol. 7, Pg. 225, 1984. | |
| dog | LDLo | oral | 500mg/kg (500mg/kg) | "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." Vol. 4, Pg. 1366, 1935. | |
| infant | TDLo | unreported | 15mg/kg (15mg/kg) | "Toxicology of Drugs and Chemicals," Deichmann, W.B., New York, Academic Press, Inc., 1969Vol. -, Pg. 390, 1969. | |
| monkey | LDLo | intravenous | 10mg/kg (10mg/kg) | "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." Vol. 4, Pg. 1366, 1935. | |
| rabbit | LDLo | oral | 1gm/kg (1000mg/kg) | "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." Vol. 4, Pg. 1366, 1935. | |
| man | TDLo | subcutaneous | 28uL/kg (0.028mL/kg) | skin and appendages (skin): "dermatitis, other: after systemic exposure" | British Journal of Clinical Practice. Vol. 28, Pg. 289, 1974. |
| mouse | LD50 | intraperitoneal | 150mg/kg (150mg/kg) | National Technical Information Service. Vol. AD691-490, | |
| rat | LD50 | intravenous | 1250mg/kg (1250mg/kg) | Arzneimittel-Forschung. Drug Research. Vol. 18, Pg. 676, 1968. | |
| mouse | LD50 | intravenous | 77mg/kg (77mg/kg) | Cesko-Slovenska Farmacie. Vol. 12, Pg. 94, 1963. | |
| rat | LD50 | oral | 1180mg/kg (1180mg/kg) | "Prehled Prumyslove Toxikologie; Organicke Latky," Marhold, J., Prague, Czechoslovakia, Avicenum, 1986Vol. -, Pg. 1334, 1986. | |
| rat | LD50 | subcutaneous | 190mg/kg (190mg/kg) | Drugs in Japan Vol. -, Pg. 1185, 1990. | |
| cat | LDLo | intravenous | 41mg/kg (41mg/kg) | Annals of Internal Medicine. Vol. 7, Pg. 738, 1933. | |
| mouse | LD50 | oral | 3500mg/kg (3500mg/kg) | Cesko-Slovenska Farmacie. Vol. 12, Pg. 94, 1963. | |
| rat | LD50 | intraperitoneal | 180mg/kg (180mg/kg) | Naunyn-Schmiedeberg's Archiv fuer Experimentelle Pathologie und Pharmakologie. Vol. 204, Pg. 288, 1947. | |
| 105504-42-5 | 12262-49-6 | 1341-90-8 |
| 152071-32-4 | 3,7-Bis(dimethylamino)-5-phenothiazinium Chloride | 3,7-Bis(dimethylamino)phenazathionium chloride |
| 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride | 3,7-bis(dimethylamino)-phenothiazin-5-ium chloride salt | 3H-Phenothiazine, 3-methochloride |
| 3H-Phenothiazine, 7-(dimethylamino)-3-(methylimino)-, 3-methochloride | 61-73-4 | 6476-03-5 |
| 8NAP7826UB | 97130-83-1 | A0574 |
| AC-15225 | AK326199 | AKOS000486124 |
| AKOS015916406 | AS-35256 | Aizen Methylene Blue BH |
| Aizen Methylene Blue FZ | BIA4084 | Basic Blue 9;Tetramethylthionine chloride |
| Basic blue 9 | Bleu de methylene | C.I. 52 015 |
| C.I. 52015 | C.I. Basic Blue 9 | C.I. Basic Blue 9 (8CI) |
| C00220 | C16H20N3S.HCl | CAS-61-73-4 |
| CCRIS 833 | CHEBI:6872 | CHEMBL405110 |
| CI 52015 | CI Basic Blue 9 | CI NO 52015 |
| CI-52015 | CS-2959 | CXKWCBBOMKCUKX-UHFFFAOYSA-M |
| Calcozine Blue ZF | Caswell No. 567 | Ceruleum methylenum |
| Chlorure de methylthioninium | Chlorure de methylthioninium [INN-French] | Chromosmon |
| Cloruro de metiltioninio | Cloruro de metiltioninio [INN-Spanish] | D And C Blue Number 1 |
| D and C Blue No. 1 | DB09241 | DOG (COUCH) GRASS Powder |
| DSSTox_CID_3296 | DSSTox_GSID_23296 | DSSTox_RID_76963 |
| DTXSID0023296 | EINECS 200-515-2 | EPA Pesticide Chemical Code 039505 |
| Ehrlichs reagent III | Ext D and C Blue No. 1 | External Blue 1 |
| FT-0622580 | HMS2598O03 | HSDB 1405 |
| HY-14536 | Hidaco Methylene Blue Salt Free | KS-000013GW |
| KS-00004B65 | LS-268 | Leather Pure Blue HB |
| Loefflers Methylene Blue | Lowacryl blue 9 | M-B Tabs |
| M0501 | MCULE-7744087830 | METHYLENE BLUE (METHYLENE BLUE, CHLORIDE:HYDRATE (7220-79-3)) |
| MFCD00012111 | MLS000719838 | Methylenblau |
| Methylenblau [German] | Methylene Azure II | Methylene Blue (C.I. 52015) |
| Methylene Blue (Inhibitor) | Methylene Blue (Medicinal) | Methylene Blue (anhydrous) |
| Methylene Blue 2B | Methylene Blue 2BF | Methylene Blue 2BN |
| Methylene Blue 2BP | Methylene Blue A | Methylene Blue B |
| Methylene Blue BB | Methylene Blue BB (Zinc Free) | Methylene Blue BBA |
| Methylene Blue BD | Methylene Blue BP | Methylene Blue BPC |
| Methylene Blue BX | Methylene Blue BZ | Methylene Blue Chloride (Biological stain) |
| Methylene Blue D | Methylene Blue FZ | Methylene Blue G |
| Methylene Blue GZ | Methylene Blue HGG | Methylene Blue I (Medicinal) |
| Methylene Blue IAD | Methylene Blue JFA | Methylene Blue N |
| Methylene Blue NF (Medicinal) | Methylene Blue NZ | Methylene Blue SG |
| Methylene Blue SP | Methylene Blue USP (Medicinal) | Methylene Blue USP XII (Medicinal) |
| Methylene Blue ZF | Methylene Blue ZX | Methylene Blue Zinc Free |
| Methylene Blue anhydrous | Methylene Blue chloride | Methylene Blue polychrome |
| Methylene Blue, pure, certified, residual water | MethyleneBlueHydrate | Methylenium ceruleum |
| Methylenum coeruleum | Methylthionine chloride | Methylthionini chloridum |
| Methylthioninii chloridum | Methylthioninii chloridum [INN-Latin] | Methylthioninium chloride |
| Methylthioninium chloride [INN] | Methylthionium chloride | Metiltioninio cloruro |
| Metiltioninio cloruro [DCIT] | Mitsui Methylene Blue | Modr Methylenova [Czech] |
| Modr Rozpoustedlova 8 [Czech] | Modr Zasadita 9 [Czech] | Modr methylenova |
| Modr rozpoustedlova 8 | Modr zasadita 9 | N-(7-(dimethylamino)-3H-phenothiazin-3-ylidene)-N-methylmethanaminium chloride |
| N-[7-(dimethylamino)-3H-phenothiazin-3-ylidene]-N-methylmethanaminium chloride | NCGC00167496-01 | NCGC00167496-03 |
| NCGC00255351-01 | NSC 215213 | NSC-215213 |
| NSC-3089 | NSC-617593 | NSC-759135 |
| NSC215213 | NSC3089 | NSC617593 |
| NSC759135 | Pharmakon1600-01505444 | Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride |
| Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride (1:1) | Phenothiazin-5-ium,7-bis(dimethylamino)-, chloride | Prestwick_326 |
| Provayblue | Q422134 | Rember |
| SBB060907 | SCHEMBL1351 | SMR000304367 |
| ST093058 | ST50406802 | STK018918 |
| Sandocryl Blue BRL | Schultz No. 1038 | Solvent blue 8 |
| Swiss Blue | Tetramethylene Blue | Tetramethylthionine chloride |
| Tox21_112497 | Tox21_112497_1 | Tox21_302087 |
| UNII-8NAP7826UB | Urolene blue | WLN: T C666 BS EY INJ EUK1&1 MN1&1 &G &421 |
| X 138 | Yamamoto Methylene Blue B | Yamamoto Methylene Blue ZF |
| [7-(dimethylamino)dibenzo[b,e]1,4-thiazin-3-yl]dimethylamine, chloride | [7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium;chloride | [7-(dimethylamino)phenothiazin-3-ylidene]dimethylamine, chloride |
| azul de metileno | methylene blue | methylene-blue |
| s4535 |
| DrugBank Name | Methylene blue |
| DrugBank | DB09241 |
| CAS Number | 105504-42-5, 12262-49-6, 1341-90-8, 152071-32-4, 37247-10-2, 61-73-4, 6476-03-5, 7220-79-3, 97130-83-1 |
| PubChem Compound | 6099 |
| KEGG Compound ID | C00220 |
| KEGG Drug | D10537 |
| PubChem.Substance | 310265144 |
| ChEBI | 6872 |
| PharmGKB | PA450457 |
| ChemSpider | 5874 |
| Wikipedia | Methylene blue |