D1297 | Antipyrine
S
N
S02DA03 Phenazone
[S02DA] Analgesics and anesthetics
[S02D] OTHER OTOLOGICALS
[S02] OTOLOGICALS
[S] Sensory organs
N02BB71 Phenazone, combinations with psycholeptics
[N02BB] Pyrazolones
[N02B] OTHER ANALGESICS AND ANTIPYRETICS
[N02] ANALGESICS
[N] Nervous system
N02BB51 Phenazone, combinations excl. psycholeptics
[N02BB] Pyrazolones
[N02B] OTHER ANALGESICS AND ANTIPYRETICS
[N02] ANALGESICS
[N] Nervous system
N02BB01 Phenazone
[N02BB] Pyrazolones
[N02B] OTHER ANALGESICS AND ANTIPYRETICS
[N02] ANALGESICS
[N] Nervous system
| Toxicity | Dose | Time | Species | Model | Method | Action | Positive criterion | Reference |
|---|---|---|---|---|---|---|---|---|
| MEMBRANE POTENTIAL | 300 µM | 30 mins | mouse | liver mitochondria | Rh123 fluorescence (excitation 485 nm, emission 535 nm) are recorded using a fluorescence multi-well plate reader (mCICCP (20 µM) treatments was considered as the 100% baseline for ΔΨm loss) | decrease | EC20 | 36 |
| RESPIRATION | > 400 µM | 60 mins | mouse | liver mitochondria | Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition. | decrease | EC20 | 36 |
| RESPIRATION | > 400 µM | 60 mins | mouse | liver mitochondria | Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Oligomycin A (1µM) was used as 100% baseline for complex II inhibition. | decrease | EC20 | 36 |
| SWELLING | > 400 µM | 30 mins | mouse | liver mitochondria | swelling assay: Absorbance at 545 nm using a fluorescence multi-well plate reader (CaCl2 (50 µM) was considered as the 100% baseline for the swelling ) | increase | EC20 | 36 |
| Target | Dose | Time | Species | Model | Method | Action | Positive criterion | Reference |
|---|---|---|---|---|---|---|---|---|
| NADH:ubiquinone reductase | > 400 µM | 60 mins | mouse | liver mitochondria | Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition. | inhibit | EC20 | 36 |
| Succinate dehydrogenase | > 400 µM | 60 mins | mouse | liver mitochondria | Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Oligomycin A (1µM) was used as 100% baseline for complex II inhibition. | inhibit | EC20 | 36 |
| Cytochrome c | > 400 µM | 30 mins | mouse | liver mitochondria | Cytochrome c release was evaluated using ELISA kit ( 20 µg/ml Alamethicin was used as 100% baseline) | release | EC20 | 36 |
| Pictogram | Signal | Statements | Precautionary Statement Codes |
|---|---|---|---|
 |
Warning |
Aggregated GHS information provided by 307 companies from 14 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies. Reported as not meeting GHS hazard criteria by 1 of 307 companies. For more detailed information, please visit ECHA C&L website Of the 13 notification(s) provided by 306 of 307 companies with hazard statement code(s): H302 (100%): Harmful if swallowed [Warning Acute toxicity, oral] H315 (73.86%): Causes skin irritation [Warning Skin corrosion/irritation] H317 (68.63%): May cause an allergic skin reaction [Warning Sensitization, Skin] H319 (73.86%): Causes serious eye irritation [Warning Serious eye damage/eye irritation] H335 (73.53%): May cause respiratory irritation [Warning Specific target organ toxicity, single exposure Respiratory tract irritation] Information may vary between notifications depending on impurities, additives, and other factors. The percentage value in parenthesis indicates the notified classification ratio from companies that provide hazard codes. Only hazard codes with percentage values above 10% are shown. |
P261, P264, P270, P271, P272, P280, P301+P312, P302+P352, P304+P340, P305+P351+P338, P312, P321, P330, P332+P313, P333+P313, P337+P313, P362, P363, P403+P233, P405, and P501; (The corresponding statement to each P-code can be found at the GHS Classification page.) |
| Organism | Test type | Route | Dose (normalized dose) | Effect | Source |
|---|---|---|---|---|---|
| mouse | LD50 | intraperitoneal | 982mg/kg (982mg/kg) | Journal of the American Pharmaceutical Association, Scientific Edition. Vol. 45, Pg. 137, 1956. | |
| mouse | LD50 | oral | 1437mg/kg (1437mg/kg) | Journal of the American Pharmaceutical Association, Scientific Edition. Vol. 45, Pg. 137, 1956. | |
| rat | LD50 | oral | 1338mg/kg (1338mg/kg) | Journal of the American Pharmaceutical Association, Scientific Edition. Vol. 45, Pg. 137, 1956. | |