F021215 | Energy charge
| Compound | Dose | Time | Species | Model | Method | Action | Result | Positive criterion | Reference |
|---|---|---|---|---|---|---|---|---|---|
| Berberine | 25 μM | 48, 72 hr | K1735-M2 mouse melanoma cells | An acid extraction procedure was used to evaluate the intracellular concentration of adenine nucleotides. Adenine nucleotides were separated by reverse-phase high-performance liquid chromatography. Quantification was achieved by employing ATP, ADP, and AMP standard curves. | decrease | Positive | p< 0.05 vs. control for each time of incubation | 327 | |