|48, 72 hr
|K1735-M2 mouse melanoma cells
|An acid extraction procedure was used to evaluate the intracellular concentration of adenine nucleotides. Adenine nucleotides were separated by reverse-phase high-performance liquid chromatography. Quantification was achieved by employing ATP, ADP, and AMP standard curves.
|p< 0.05 vs. control for each time of incubation