DS16570511 |
7 μM |
|
|
HEK293A cells |
HEK293A cell lines expressing the mitochondria-targeted Ca2+ indicator protein aequorin |
Positive |
IC50 |
302 |
SB202190 |
10 μM |
|
|
intact and in permeabilized HeLa cells (MM5 cells expressing mitmutAEQ ) |
mitochondrial Ca2+ uptake |
Positive |
|
233 |
kaempferol |
1μM |
|
|
|
The HeLa cell clone MM5 expressing mitochondrially targeted mutated aequorin was used for [Ca2+]M measurements. [Ca2+]c measurements were carried out using HeLa cells transiently transfected with the plasmid for cytosolic aequorin. |
Positive |
|
234 |
kaempferol |
7μM |
|
|
|
The HeLa cell clone MM5 expressing mitochondrially targeted mutated aequorin was used for [Ca2+]M measurements. [Ca2+]c measurements were carried out using HeLa cells transiently transfected with the plasmid for cytosolic aequorin. |
Positive |
EC50 |
234 |
quercetin |
21 μM |
|
|
|
mitochondrial Ca2+ uptake |
Positive |
EC50 |
234 |
genistein |
37μM |
|
|
|
mitochondrial Ca2+ uptake |
Positive |
EC50 |
234 |
kaempferol |
10 μmol/L |
|
|
periodically paced rat ventricular myocytes (VMs) under β-adrenergic stimulation |
mitochondrial Ca2+ monitored by mitochondrial-targeted Ca2+ biosensor mtRCamp1h. |
Positive |
|
241 |
Ru360 |
2 μmol/L |
|
|
|
|
Positive |
|
241 |