Compound	Dose	Time	Species	Model	Method	Action	Result	Positive criterion	Reference
Melittin	5μM and 16μM		bovine	bovine heart MF1-ATPase			Positive	IC50	170
Nimesulide	1 mM		rat; Sprague-Dawley Crl:CD (SD) BR	lung microsomes	measurement of NADPH oxidation	increase	Positive	p < 0.05	11
IF1	0.25 μM		bovine	bovine heart MF1-ATPase			Positive	IC50	170
IF1	1.2 μM		bovine	heart MF1-ATPase			Positive	IC50	170
IF1	0.84 μM		bovine	heart MF1-ATPase			Positive	IC50	170
IF1	300 μg/mg protein (T. pyriformis SMP-ATPase) ;						Positive	IC50	170
IF1	34 μg/mg protein		C. asciculate	SMP-ATPase			Positive	IC50	170
IF1	0.24 μM		rat	liver MF1-ATPase			Positive	Ki	170
Enterostatin							Positive		170
ethyldimethylaminopropyl carbodiimide	13 mol of EDC/mol F1 (EF1-ATPase)						Positive	95% inhibition	170
1,5-difluoro-2,4-dinitrobenzene							Positive		170
Angiostatin							Positive		170
NBD-Cl							Positive		170
NBD-Cl							Positive		170
Gentamicin	300 μg/ml	1hr	postnatal day 6 (P6±1d) FVB mice	basal turn outer hair cells ( low-frequency processing OHCs) from intact cochlear (organ of Corti) explants	scanning confocal microscope (NADH autofluorescence); T1 imaging buffer contained 5 mM glucose. T2 imaging buffer contained 10 mM glucose, 3 mM glutamate and 2 mM pyruvate.	decrease	Positive		312
Troglitazone	>25 µM	1 and 2 hr		HepG2 cells	Cellular ATP concentrations were measured with a ATP Bioluminescent Somatic Cell Assay Kit	decrease	Positive		332
Amitriptyline							Positive		197
Desipramine							Positive		197
lumiracoxib							Positive		197
Berberine	25 μM	48, 72 hr		K1735-M2 mouse melanoma cells	An acid extraction procedure was used to evaluate the intracellular concentration of adenine nucleotides. Adenine nucleotides were separated by reverse-phase high-performance liquid chromatography. Quantification was achieved by employing ATP, ADP, and AMP standard curves.	decrease	Positive	p< 0.05 vs. control for each time of incubation	327
Berberine	10 μM	24 hr		K1735-M2 mouse melanoma cells	An acid extraction procedure was used to evaluate the intracellular concentration of adenine nucleotides. Adenine nucleotides were separated by reverse-phase high-performance liquid chromatography. Quantification was achieved by employing ATP, ADP, and AMP standard curves.	decrease	Positive	p< 0.05 vs. control for each time of incubation	327
buformin	62.5 μM	24 hours	human	HepG2 cells	Assay of Cellular ATP Content	decrease	Positive	p < 0.001	15
buformin	62.5 μM	24 hours	human	HepG2 cells	Assay of Cellular ATP Content	decrease	Positive	p < 0.001	15
Phenformin	62.5 μM	24 hours	human	HepG2 cells	Assay of Cellular ATP Content	decrease	Positive	p < 0.001	15
Phenformin	62.5 μM	24 hours	human	HepG2 cells	Assay of Cellular ATP Content	decrease	Positive	p < 0.001	15
Metformin	657 μM	24 hours	human	hepatocytes	Assay of Cellular ATP Content	decrease	Positive	IC50	15
buformin	26.1 μM	24 hours	human	hepatocytes	Assay of Cellular ATP Content	decrease	Positive	IC50	15
Phenformin	11.7 μM	24 hours	human	hepatocytes	Assay of Cellular ATP Content	decrease	Positive	IC50	15
Metformin	1430 μM	24 hours	human	HepG2 cells	Assay of Cellular ATP Content	decrease	Positive	IC50	15
buformin	43.7 μM	24 hours	human	HepG2 cells	Assay of Cellular ATP Content	decrease	Positive	IC50	15
Phenformin	12.9 μM	24 hours	human	HepG2 cells	Assay of Cellular ATP Content	decrease	Positive	IC50	15
Metformin	> 2000 μM	24 hours	human	HepG2 cells	Assay of Cellular ATP Content	decrease	Positive	IC50	15
buformin	> 500 μM	24 hours	human	HepG2 cells	Assay of Cellular ATP Content	decrease	Positive	IC50	15
Phenformin	> 500 μM	24 hours	human	HepG2 cells	Assay of Cellular ATP Content	decrease	Positive	IC50	15
Diazepam	150μg/ml	2hr		PE cells	luciferin-luciferase method	decrease	Positive		293
Troglitazone			human	hepatocytes	ATP bioluminescence assay	decrease	Positive		226
Diclofenac	50 μmol/L	10 minutes	rat	liver mitochondria	Measurement of Mitochondrial NADPH; incubated in the presence of Ca2+	increase	Positive	p < 0.01	6
Diclofenac	250 μmol/L	24 hours	rat	hepatocytes	Assay of Cellular ATP contents; Assay of LDH leakage	decrease	Positive	p < 0.01	6
Diclofenac	500 μmol/L	24 hours	rat	hepatocytes	Assay of Cellular ATP contents; Assay of LDH leakage	decrease	Positive	p < 0.01	6

F021201. ATP synthesis
F021202. NADPH metabolism
F021203. ATP level
F021204. ATP hydrolysis
F021205. Heme synthesis
F021206. CoA synthesis
F021207. Ubiquinone synthesis
F021208. Cobalamin (Cbl, vitamin B12) metabolism
F021209. folate (vitamin B9) metabolism
F021210. calciferol (Vitamin D) metabolism
F021211. biotin (vitamin B7) metabolism
F021212. Thiamine (vitamin B1) metabolism
F021213. Riboflavin (vitamin B2) metabolism
F021214. NADH level
F021215. energy charge
F021216. ATP/ADP ratio

T019003. F1 subunits
T019003003. ATP synthase subunit alpha, mitochondrial
T019003004. ATP synthase subunit beta, mitochondrial
T020. ATP