Compound	Dose	Time	Species	Model	Method	Result	Positive criterion	Reference
Antimycin A						Positive		35
Benoxaprofen	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Negative	p < 0.05	3
Myxothiazol						Positive		35
Bezafibrate	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Negative	p < 0.05	3
Chlorpromazine	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Positive	p < 0.001	3
Dantrolene	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Negative	p < 0.05	3
Diclofenac	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Negative	p < 0.05	3
decylubiquinol	19.4 ± 7.17 (female), 25.00 ± 10.30 (male)		zebrafish (adult, 24 wk)	isolate mitochondria from liver	isothermal titration calorimetry (ITC)	Positive	Kd	309
hexachlorobenzene			zebrafish (adult, 24 wk)		complex III activity assay: decylubiquinol-dependent reduction of cytochrome c by mitochondrial complex III.	Negative		309
Antimycin A						Positive		185
stigmatellin						Positive		185
Fluconazole	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Negative	p < 0.05	3
ethoxyformic anhydride						Positive		70
gefitinib	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Negative	p < 0.05	3
atorvastatin				C2C12 myoblasts		Positive		180
Linezolid	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Negative	p < 0.05	3
Cerivastatin	100uM			C2C12 myoblasts	measured ubiquinol:cytochrome c oxidoreductase activity in broken C2C12 mitochondria after acute statin exposure at a fixed concentration for all compounds	Positive		180
pitavastatin	100uM			C2C12 myoblasts	measured ubiquinol:cytochrome c oxidoreductase activity in broken C2C12 mitochondria after acute statin exposure at a fixed concentration for all compounds	Positive		180
lovastatin	100uM			C2C12 myoblasts	measured ubiquinol:cytochrome c oxidoreductase activity in broken C2C12 mitochondria after acute statin exposure at a fixed concentration for all compounds	Positive		180
Simvastatin	100uM			C2C12 myoblasts	Measured ubiquinol:cytochrome c oxidoreductase activity in broken C2C12 mitochondria after acute statin exposure at a fixed concentration for all compounds. The lactone forms of the indicated statins were included in the assay medium at their cytotoxic EC50 concentration for measurement of their effect on the catalytic capacity.	Positive		180
Metformin	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Negative	p < 0.05	3
tacrolimus	1 μM		rat	isolated kidney mitochondria	Mitochondrial complex III assay (ubiquinol‐cytochrome c oxidoreductase)	Positive	30%	323
rapamycin	1 μM		rat	isolated kidney mitochondria	Mitochondrial complex III assay (ubiquinol‐cytochrome c oxidoreductase)	Positive	24%	323
Simvastatin	200uM		bovine	heart mitochondria	Reduction of the CIII cytochromes c1 and b was determined spectrophotometrically in bovine heart mitochondria.	Positive	p < 0.01	180
Nefazodone	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Positive	p < 0.01	3
paroxetine	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Positive	p < 0.001	3
Myxothiazol						Positive		81
moas						Positive		82
azoxystrobin						Positive		83
oudemansin a						Positive		84
pravastatin	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Negative	p < 0.05	3
strobilurin a						Positive		84
cpmb-oxime						Positive		84
azoxystrobin	100 mM	incubated overnight	bovine	isolated heart mitochondria	Measurements of redox potentials of the ISP	Positive		83
famoxadone	100 mM	incubated overnight	bovine	isolated heart mitochondria	Measurements of redox potentials of the ISP	Positive		83
Primaquine	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Negative	p < 0.05	3
stigmatellin	100 mM	incubated overnight	bovine	isolated heart mitochondria	Measurements of redox potentials of the ISP	Positive		83
uhdbt	100 mM	incubated overnight	bovine	isolated heart mitochondria	Measurements of redox potentials of the ISP	Positive		83
hhdbt uhnq	100 mM	incubated overnight	bovine	isolated heart mitochondria	Measurements of redox potentials of the ISP	Positive		83
dibromothymoquinone	100 mM	incubated overnight	bovine	isolated heart mitochondria	Measurements of redox potentials of the ISP	Positive		83
radicicol	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Negative	p < 0.05	3
NQNO			bovine	isolated heart mitochondria cytochrome bc1 complex		Positive		166
sertraline						Positive		307
Fluoxetine						Positive		307
Antimycin A			bovine	isolated heart mitochondria cytochrome bc1 complex		Positive		166
Rosiglitazone	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Negative	p < 0.05	3
diuron						Positive		83
NQNO			bovine	isolated heart mitochondria cytochrome bc1 complex		Positive		166
Antimycin A						Positive		26
Simvastatin	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Positive	p < 0.001	3
ethylxanthate						Positive		167
bisulfate						Positive		167
semicarbazide						Positive		167
Tamoxifen	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Positive	p < 0.001	3
salicyaldoxime						Positive		167
3-NPA				permeabilized LUHMES cells	Assessment of the function of individual mitochondrial complexes using Agilent Seahorse XFe24	Positive	EC25(NA) [Glc/Gal]	326
Antimycin A	12000			LUHMES (Lund human mesencephalic) cells	Glc–Gal–NeuriTox assay	Positive	EC25(NA) [Glc/Gal]	326
azoxystrobin	3.6			LUHMES (Lund human mesencephalic) cells	Glc–Gal–NeuriTox assay	Positive	EC25(NA) [Glc/Gal]	326
trazodone	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Negative	p < 0.05	3
Myxothiazol			bovine	heart mitochondria		Positive		29
Leflunomide	50 μM	48 hr		MEF cells	Mitochondrial Complex III Activity Assay kit(BioVision)	Positive		295
Trovafloxacin	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Negative	p < 0.05	3
Paraquat	250 μM	5min	Sprague Dawley rat	brain cultures		Positive		242
Maneb	30 mg/kg i.p. twice a week/6 weeks		C57BL/6 mice			Positive		242
Tamoxifen	26.6 μM		bovine	heart mitochondria	Measurement of complex IV activity	Positive	IC50	3
Tamoxifen	30 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Positive	IC50	3
Aluminium	10 mg/kg/day intragastric for 12 weeks		Wistar rats			Positive		242
paroxetine						Positive		35
Pioglitazone						Positive		35
promethazine						Positive		35
Tamoxifen						Positive		35
picoxystrobin	50µM			permeabilized LUHMES cells	Assessment of the function of individual mitochondrial complexes using Agilent Seahorse XFe24	Positive		326
pyraclostrobin	50µM			permeabilized LUHMES cells	Assessment of the function of individual mitochondrial complexes using Agilent Seahorse XFe24	Positive		326
Amiodarone	50 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Positive	p < 0.001	3

T004001. Cytochrome b
T004002. Cytochrome b-c1 complex subunit 2, mitochondrial
T004003. Cytochrome b5 outer mitochondrial membrane isoform
T004004. Cytochrome b-c1 complex subunit 7
T004005. Cytochrome c1, heme protein, mitochondrial
T004006. Mitochondrial chaperone BCS1
T004007. Mitogen-activated protein kinase kinase kinase 20
T004008. Tetratricopeptide repeat protein 19, mitochondrial
T004009. Complex III assembly factor LYRM7
T004010. Ubiquinol-cytochrome-c reductase complex assembly factor 2
T004011. Ubiquinol-cytochrome-c reductase complex assembly factor 3
T004012. Qo site (Qp site or ubiquinol oxidation site)
T004013. Qi site (Qn site or quinone reduction site)
T004016. Cytochrome b-c1 complex subunit Rieske, mitochondrial
T004017. Cytochrome b-c1 complex subunit 8
T004018. Cytochrome b-c1 complex subunit 10
T004019. Cytochrome b-c1 complex subunit 9
T004020. Cytochrome b-c1 complex subunit 6, mitochondrial
T004021. Cytochrome b-c1 complex subunit 1, mitochondrial

F020103. electron transport chain
F020403. glucose galactose IC50 ratio
F0504. ROS production