Compound	Dose	Time	Species	Model	Method	Action	Result	Positive criterion	Reference
tacrolimus	1 μM		rat	isolated kidney mitochondria	Oxygen consumption was measured with a Clark‐type electrode	decrease	Positive		323
Amiodarone	10 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
Amiodarone	20 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
Amiodarone	50 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
Amiodarone	100 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
fluoranthene	100 µg/l		zebrafish		XFe24 Extracellular Flux Analyzer	increase	Positive		85
rapamycin	1 μM		rat	isolated kidney mitochondria	Oxygen consumption was measured with a Clark‐type electrode	decrease	Positive		323
benzbromarone	1 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
benzbromarone	10 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
benzbromarone	100 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
9,10-anthraquinone	20 µM		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		86
1,9-benz-10-anthrone	10 µM		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		86
2‐Butylbenzofuran	50 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.05	4
2‐Butylbenzofuran	100 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
Amiodarone	10 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
Amiodarone	20 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
Amiodarone	50 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
Amiodarone	100 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
Cisplatin						decrease	Positive		307
Cyclophosphamide						decrease	Positive		307
benzarone	20 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
benzarone	50 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
benzarone	100 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
Gentamicin	5mM	10min	rat	isolated rat liver (and kidney) mitochondria	Oroboros O2K Oxygraph	reduce	Positive		316
benzbromarone	0.1 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
benzbromarone	1 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
benzbromarone	10 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
benzbromarone	100 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
Berberine	1-10 µM			isolated rat muscle mitochondria	Clark type oxygen electrode (Strathkelvin Instruments)	decrease	Positive		328
ionomycin			rat	liver mitochondria	Oxygen consumption and medium pH were monitored simultaneously with a dual channel Gilson oxygraph equipped with a Clark electrode (Yellow Springs Instruments) and a combination pH electrode (Beckman No. 39030).	increase	Positive		202
2‐Butylbenzofuran	100 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
triclosan	0.5 µM		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		85
triclosan	30 μM		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		89
Rotenone	263±43 nM	2 minutes	human	HepG2	Measurement of OCR	decrease	Positive	EC50	7
Ciglitazone	> 100 μM	2 minutes	human	HepG2	Measurement of ECAR	decrease	Positive	EC50	7
Antimycin A	4±0.2 nM	2 minutes	human	HepG2	Measurement of OCR	decrease	Positive	EC50	7
Oligomycin	0.92± 0.18 μM	2 minutes	human	HepG2	Measurement of OCR	decrease	Positive	EC50	7
FCCP	54±7 nM	2 minutes	human	HepG2	Measurement of OCR	increase	Positive	EC50	7
2-Deoxy-D-glucose	150 mM	2 minutes	human	HepG2	Measurement of OCR	increase	Positive	EC50	7
Tolcapone	3.7 μM	2 minutes	human	HepG2	Measurement of OCR	increase	Positive	EC50	7
Entacapone	33 μM	2 minutes	human	HepG2	Measurement of OCR	increase	Positive	EC50	7
Nilutamide	66 μM	2 minutes	human	HepG2	Measurement of OCR	decrease	Positive	EC50	7
Flutamide	> 100 μM	2 minutes	human	HepG2	Measurement of OCR	decrease	Positive	EC50	7
Chlorpromazine	> 300 μM	2 minutes	human	HepG2	Measurement of OCR	increase	Positive	EC50	7
Diflunisal	15 μM	2 minutes	human	HepG2	Measurement of OCR	increase	Positive	EC50	7
2,4-dinitrophenol	0.5 μM / 24 hpf		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		90
Benoxaprofen	30 μM	2 minutes	human	HepG2	Measurement of ECAR	decrease	Positive	EC50	7
Troglitazone	> 100 μM	2 minutes	human	HepG2	Measurement of ECAR	decrease	Positive	EC50	7
Troglitazone	10-50 μM		ZDF fa/fa rat & ZDF lean rat	isolated liver mitochondria	OCR and measured using a fluorescent oxygen probe (Presens)	decrease	Positive	significantly different from control (p < 0.05)	225
Pioglitazone	25-50 μM		ZDF fa/fa rat & ZDF lean rat	isolated liver mitochondria	OCR and measured using a fluorescent oxygen probe (Presens)	decrease	Positive	significantly different from control (p < 0.05)	225
Troglitazone	10 μM	2 minutes	feline	cardiomyocytes	Measurement of OCR	increase	Positive	EC50	7
Ciglitazone	> 100 μM	2 minutes	feline	cardiomyocytes	Measurement of OCR	decrease	Positive	EC50	7
Rosiglitazone	> 100 μM	2 minutes	feline	cardiomyocytes	Measurement of OCR	increase	Positive	EC50	7
Yohimbine			mouse	neuron	Oxygen consumption rate (OCR) or extracellular acidification rate (ECAR) measurements from permeabilized neurons and isolated brain mitochondria were performed using the XF96 Extracellular Flux analyzer (Seahorse Bioscience).	increase	Positive		269
Dyclonine			mouse	neuron	Oxygen consumption rate (OCR) or extracellular acidification rate (ECAR) measurements from permeabilized neurons and isolated brain mitochondria were performed using the XF96 Extracellular Flux analyzer (Seahorse Bioscience).	increase	Positive		269
Metformin	2mM	30 minutes	mouse; C57BL/6J	isolated skeletal muscle mitochondria	Measurement of Oxygen level; incubated in complex I substrate; added FCCP (1.5 μM)	decrease	Positive	p < 0.05	172
Nimesulide	1 mM		rat; Sprague-Dawley Crl:CD (SD) BR	lung microsomes	measurement of oxygen consumption	increase	Positive	p < 0.05	11
tacrolimus	50 µg/ml	12hr		INS-1 cells	XF24 Extracellular Flux Analyzer	decrease	Positive		322
tacrolimus	50 µg/ml	12hr		INS-1 cells	XF24 Extracellular Flux Analyzer	decrease	Positive		322
tacrolimus	50 µg/ml	12hr		INS-1 cells	XF24 Extracellular Flux Analyzer	decrease	Positive		322
tacrolimus	50 µg/ml	12hr		INS-1 cells	XF24 Extracellular Flux Analyzer	decrease	Positive		322
tacrolimus	1 μM		rat	isolated kidney mitochondria	Oxygen consumption was measured with a Clark‐type electrode	decrease	Positive	10%	323
rapamycin	1 μM		rat	isolated kidney mitochondria	Oxygen consumption was measured with a Clark‐type electrode	decrease	Positive	11%	323
tacrolimus	3.4E−11 M and 2.3E−8 M		rat	isolated kidney mitochondria	Oxygen consumption was measured with a Clark‐type electrode	decrease	Positive	EC50 ; maximal inhibitory effect was about 20%	323
rapamycin	4.4×10−10 M		rat	isolated kidney mitochondria	Oxygen consumption was measured with a Clark‐type electrode	decrease	Positive	EC50 ; maximal inhibitory effect was about14%	323
triclosan	0.5 µM		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		85
triclosan	0.5 µM		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		85
Benzo(A)Pyrene	100 µg/l		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		85
Benzo(A)Pyrene	1000 µg/l		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		85
phenanthrene	500 µg/l		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		85
phenanthrene	100 µg/l		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		85
fluoranthene	100 µg/l		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		85
fluoranthene	100 µg/l		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		85
fluoranthene	100 µg/l		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		85
phenanthrene	500 µg/l		zebrafish		XFe24 Extracellular Flux Analyzer	increase	Positive		85
Phenanthrenequinone	2 µM		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		86
benz(a)anthracene-7,12-dione	5 µM		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		86
Phenanthrenequinone	2 µM		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		86
xanthone	20 µM		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		86
benz(a)anthracene-7,12-dione	5 µM		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		86
1,9-benz-10-anthrone	10 µM		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		86
9,10-anthraquinone	20 µM		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		86
1,9-benz-10-anthrone	10 µM		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		86
xanthone	20 µM		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		86
buformin	25 μM	24 hours	human	HepG2 cells	Measurement of OCR	decrease	Positive	p < 0.001	15
Phenformin	25 μM	24 hours	human	HepG2 cells	Measurement of OCR	decrease	Positive	p < 0.001	15
2,4-dinitrophenol	0.5 μM / 6 hpf		zebrafish		XFe24 Extracellular Flux Analyzer	increase	Positive		90
2,4-dinitrophenol	0.5 μM / 48 hpf		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		90
2,4-dinitrophenol	0.5 μM / 24 hpf		zebrafish		XFe24 Extracellular Flux Analyzer	affect	Positive		90
2,4-dinitrophenol	0.5 μM / 48 hpf		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		90
triclosan	30 μM		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		89
2,4-dinitrophenol	0.5 μM / 24 hpf		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		90
triclosan	30 μM		zebrafish		XFe24 Extracellular Flux Analyzer	increase	Positive		89
quercetin	1 µg/l		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		91
quercetin	1 µg/l		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		91
quercetin	10 µg/l		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		91
2,4-dinitrophenol	0.5 μM / 24 hpf		zebrafish		XFe24 Extracellular Flux Analyzer	increase	Positive		90
diquat	100 μM		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		92
diquat	100 μM		zebrafish		XFe24 Extracellular Flux Analyzer	decrease	Positive		92

F02010201. respiratory control ratio (RCR)
F02010202. oxygen consumption rate (OCR)
F02010203. basal respiration
F02010204. maximal respiration
F02010205. ATP turnover
F02010206. spare/ respiratory capacity
F02010207. proton leak
F02010208. non-mitochondrial respiration
F02010209. P/O ratio ( ADP/O)

T001. NADH:ubiquinone reductase