F07 | Cell death

GO:0008219
Cell death

Compound Dose Time Species Model Method Action Result Positive criterion Reference
Fluorouracil 0.5 - 800 μM Colo320; DLD1; HT29; LoVo; SW480 increase Positive 164
Nimesulide 8 24hr rat hepatocytes DNA damage 153 induction (GADD153 antibodies) for ER-stress induced apoptosis induce Positive AC50 (μM) 40
Bafilomycin A1 inhibit Positive 200
Bafilomycin A1 pediatric B ALL cells induce Positive 200
Bafilomycin A1 induce Positive 200
FOX-988 increase Positive 28
Sdz-51641 increase Positive 28
Flufenamic Acid 68 24hr rat hepatocytes DNA damage 153 induction (GADD153 antibodies) for ER-stress induced apoptosis induce Positive AC50 (μM) 40
Tolfenamic Acid 50 24hr rat hepatocytes DNA damage 153 induction (GADD153 antibodies) for ER-stress induced apoptosis induce Positive AC50 (μM) 40
Mefenamic acid 61 24hr rat hepatocytes DNA damage 153 induction (GADD153 antibodies) for ER-stress induced apoptosis induce Positive AC50 (μM) 40
Meloxicam 153 24hr rat hepatocytes DNA damage 153 induction (GADD153 antibodies) for ER-stress induced apoptosis induce Positive AC50 (μM) 40
Sudoxicam 223 24hr rat hepatocytes DNA damage 153 induction (GADD153 antibodies) for ER-stress induced apoptosis induce Positive AC50 (μM) 40
Piroxicam 610 24hr rat hepatocytes DNA damage 153 induction (GADD153 antibodies) for ER-stress induced apoptosis induce Positive AC50 (μM) 40
Diflunisal 50 24hr rat hepatocytes DNA damage 153 induction (GADD153 antibodies) for ER-stress induced apoptosis induce Positive AC50 (μM) 40
Troglitazone human hepatocytes cell viability assay induce Positive 226
Diclofenac 51 24hr rat hepatocytes DNA damage 153 induction (GADD153 antibodies) for ER-stress induced apoptosis induce Positive AC50 (μM) 40
Sulindac sulfide 53 24hr rat hepatocytes DNA damage 153 induction (GADD153 antibodies) for ER-stress induced apoptosis induce Positive AC50 (μM) 40
Flufenamic Acid 70 24hr rat hepatocytes cytochrome c release (anti-cytochrome c antibody ) induce Positive AC50 (μM) 40
apoptolidin 18 μM 60 human cancer cell lines of the National Cancer Institute Positive mean 184
cytovaricin 1 μM 60 human cancer cell lines of the National Cancer Institute Positive mean 184
Oligomycin 0.3 μM 60 human cancer cell lines of the National Cancer Institute Positive mean 184
Tolfenamic Acid 75 24hr rat hepatocytes cytochrome c release (anti-cytochrome c antibody ) induce Positive AC50 (μM) 40
Mefenamic acid 277 24hr rat hepatocytes cytochrome c release (anti-cytochrome c antibody ) induce Positive AC50 (μM) 40
Meloxicam 245 24hr rat hepatocytes cytochrome c release (anti-cytochrome c antibody ) induce Positive AC50 (μM) 40
Sudoxicam 282 24hr rat hepatocytes cytochrome c release (anti-cytochrome c antibody ) induce Positive AC50 (μM) 40
Piroxicam 598 24hr rat hepatocytes cytochrome c release (anti-cytochrome c antibody ) induce Positive AC50 (μM) 40
Diflunisal 79 24hr rat hepatocytes cytochrome c release (anti-cytochrome c antibody ) induce Positive AC50 (μM) 40
Diclofenac 197 24hr rat hepatocytes cytochrome c release (anti-cytochrome c antibody ) induce Positive AC50 (μM) 40
Sulindac sulfide 59 24hr rat hepatocytes cytochrome c release (anti-cytochrome c antibody ) induce Positive AC50 (μM) 40
Nimesulide 21 24hr rat hepatocytes cytochrome c release (anti-cytochrome c antibody ) induce Positive AC50 (μM) 40
Amiodarone 100 μmol/L 8 hours human HepG2 Assessment of cytochrome c release increase Positive observable 4
benzarone 100 μmol/L 8 hours human HepG2 Assessment of cytochrome c release increase Positive observable 4
benzbromarone 100 μmol/L 8 hours human HepG2 Assessment of cytochrome c release increase Positive observable 4
Amiodarone 100 μmol/L 8 hours rat; Sprague–Dawley hepatocytes Apoptosis measurement increase Positive p < 0.01 4
benzarone 100 μmol/L 8 hours rat; Sprague–Dawley hepatocytes Apoptosis measurement increase Positive p < 0.01 4
benzbromarone 100 μmol/L 8 hours rat; Sprague–Dawley hepatocytes Apoptosis measurement increase Positive p < 0.01 4
Amiodarone 100 μmol/L 8 hours rat; Sprague–Dawley hepatocytes Apoptosis measurement increase Positive p < 0.05 4
benzarone 100 μmol/L 8 hours rat; Sprague–Dawley hepatocytes Apoptosis measurement increase Positive p < 0.05 4
benzbromarone 100 μmol/L 8 hours rat; Sprague–Dawley hepatocytes Apoptosis measurement increase Positive p < 0.01 4
Clotrimazole 30 μmol/L 24 hours human CAL27 Apoptosis measurement increase Positive p < 0.05 5
Clotrimazole 30 μmol/L 24 hours human SCC25 Apoptosis measurement increase Positive p < 0.05 5
Clotrimazole 30 μmol/L 24 hours human UM1 Apoptosis measurement increase Positive p < 0.05 5
Clotrimazole 40 μmol/L 24 hours human CAL27 Apoptosis measurement increase Positive p < 0.01 5
Clotrimazole 40 μmol/L 24 hours human SCC25 Apoptosis measurement increase Positive p < 0.01 5
Clotrimazole 40 μmol/L 24 hours human UM1 Apoptosis measurement increase Positive p < 0.01 5
Clotrimazole 30 μmol/L 24 hours human CAL27 Apoptosis measurement increase Positive p < 0.05 5
Etoposide isolated mitochondria increase Positive 57
Clotrimazole 30 μmol/L 24 hours human SCC25 Apoptosis measurement increase Positive p < 0.05 5
Clotrimazole 30 μmol/L 24 hours human UM1 Apoptosis measurement increase Positive p < 0.05 5
Clotrimazole 40 μmol/L 24 hours human CAL27 Apoptosis measurement increase Positive p < 0.01 5
Clotrimazole 40 μmol/L 24 hours human SCC25 Apoptosis measurement increase Positive p < 0.01 5
Clotrimazole 40 µM 12 hours human CAL27 western blot increase Positive 2.1875-fold decrease 5
Clotrimazole 40 µM 12 hours human UM1 western blot increase Positive 1.35-fold decrease 5
Clotrimazole 40 µM 24 hours human CAL27 western blot increase Positive 3.1818-fold decrease 5
Clotrimazole 40 µM 24 hours human UM1 western blot increase Positive 1.5-fold decrease 5
Clotrimazole 40 µM 48 hours human CAL27 western blot increase Positive 3.3333-fold decrease 5
Clotrimazole 40 µM 48 hours human UM1 western blot increase Positive 1.5882-fold decrease 5
Clotrimazole 40 µM 12 hours human CAL27 western blot increase Positive 1.3953-fold increase 5
Clotrimazole 40 µM 24 hours human CAL27 western blot increase Positive 1.3953-fold increase 5
Clotrimazole 40 µM 24 hours human UM1 western blot increase Positive 1.2623-fold increase 5
Clotrimazole 40 µM 48 hours human CAL27 western blot increase Positive 2.3256-fold increase 5
Clotrimazole 40 µM 48 hours human UM1 western blot increase Positive 1.6223-fold increase 5
Clotrimazole 150 mg/kg/day 6 days a week for two weeks human CAL27(xenograft tumors)/mouse Immunohistochemistry increase Positive 1.5-fold decrease 5
Clotrimazole 150 mg/kg/day 6 days a week for two weeks human CAL27(xenograft tumors)/mouse western blot analysis increase Positive 2.0-fold increase 5
p,p'-ddt 30 μM rat Sertoli cells induce Positive 242
Rotenone 0.1–0.5 nmol/mg protein Wistar rat brain isolated mitochondria Positive 242
erastin 10μM inducer Positive 245
deferoxamine 100μM inhibitor Positive 245
cycloheximide ferroptosis weak rescue inhibitor Positive 245
U0126 inhibitor Positive 245
trolox inhibitor Positive 245
N-deacetyl ketoconazole 120 μM 0.5 hours rat hepatocytes Measurement of LDH activities increase Positive p < 0.05 8
N-deacetyl ketoconazole 160 μM 0.5 hours rat hepatocytes Measurement of LDH activities increase Positive p < 0.05 8
mimosine 0.3 mM U-937 leukemia cell line. Positive 270
N-deacetyl ketoconazole 120 μM 1 hours rat hepatocytes Measurement of LDH activities increase Positive p < 0.05 8
N-deacetyl ketoconazole 160 μM 1 hours rat hepatocytes Measurement of LDH activities increase Positive p < 0.05 8
N-deacetyl ketoconazole 80 μM 2 hours rat hepatocytes Measurement of LDH activities increase Positive p < 0.05 8
N-deacetyl ketoconazole 120 μM 2 hours rat hepatocytes Measurement of LDH activities increase Positive p < 0.05 8
N-deacetyl ketoconazole 160 μM 2 hours rat hepatocytes Measurement of LDH activities increase Positive p < 0.05 8
N-deacetyl ketoconazole 60 μM 4 hours rat hepatocytes Measurement of LDH activities increase Positive p < 0.05 8
N-deacetyl ketoconazole 80 μM 4 hours rat hepatocytes Measurement of LDH activities increase Positive p < 0.05 8
N-deacetyl ketoconazole 120 μM 4 hours rat hepatocytes Measurement of LDH activities increase Positive p < 0.05 8
N-deacetyl ketoconazole 160 μM 4 hours rat hepatocytes Measurement of LDH activities increase Positive p < 0.05 8
N-deacetyl ketoconazole 120 μM 0.5 hours rat hepatocytes Measurement of LDH activities; co-treatment of 250 μM n-Octylamine increase Positive p < 0.05 8
N-deacetyl ketoconazole 120 μM 0.5 hours rat hepatocytes Measurement of LDH activities; co-treatment of 100 μM Methimazole affect Positive p < 0.05 8
N-deacetyl ketoconazole 120 μM 1 hours rat hepatocytes Measurement of LDH activities; co-treatment of 250 μM n-Octylamine increase Positive p < 0.05 8
N-deacetyl ketoconazole 120 μM 1 hours rat hepatocytes Measurement of LDH activities; co-treatment of 100 μM Methimazole affect Positive p < 0.05 8
N-deacetyl ketoconazole 160 μM 1 hours rat hepatocytes Measurement of LDH activities; co-treatment of 250 μM n-Octylamine increase Positive p < 0.05 8
N-deacetyl ketoconazole 160 μM 1 hours rat hepatocytes Measurement of LDH activities; co-treatment of 100 μM Methimazole affect Positive p < 0.05 8
Rotenone 1µM overnight HeLa cells Cyto-ID Autophagy Detection dye and Autophagy compound library in a microplate-based assay induce Positive >2 fold increase 277
nocodazole 1µM overnight HeLa cells Cyto-ID Autophagy Detection dye and Autophagy compound library in a microplate-based assay induce Positive >2 fold increase 277
Chloroquine 1µM overnight HeLa cells Cyto-ID Autophagy Detection dye and Autophagy compound library in a microplate-based assay induce Positive >2 fold increase 277
Brefeldin A 1µM overnight HeLa cells Cyto-ID Autophagy Detection dye and Autophagy compound library in a microplate-based assay induce Positive >2 fold increase 277
Tunicamycin 1µM overnight HeLa cells Cyto-ID Autophagy Detection dye and Autophagy compound library in a microplate-based assay induce Positive >2 fold increase 277
thapsigargin 1µM overnight HeLa cells Cyto-ID Autophagy Detection dye and Autophagy compound library in a microplate-based assay induce Positive >2 fold increase 277
Gingerol 1µM overnight HeLa cells Cyto-ID Autophagy Detection dye and Autophagy compound library in a microplate-based assay induce Positive >2 fold increase 277
Staurosporine 1µM overnight HeLa cells Cyto-ID Autophagy Detection dye and Autophagy compound library in a microplate-based assay induce Positive >2 fold increase 277
Clotrimazole 40 μmol/L 24 hours human UM1 Apoptosis measurement increase Positive p < 0.01 5
oxaliplatin Positive 262
MG-132 20 μM 24 hr rat PC12 cells Measurement of caspase-3 activity with ApoAlert™ CPP32/Caspase-3 assay kit increase Positive 289
MG-132 20 μM 24 hr rat PC12 cells Measurement of cytochrome c release using a solid-phase, enzyme-linked immunosorbent assay kit for the detection of cytochrome c. increase Positive 289
Troglitazone 100 µM 24hr HepG2 cells Apo-One homogeneous caspase 3 assay kit Positive 330
Niclosamide induce Positive 294
Dequalinium Chloride 24 hr Ishikawa and Caco-2 WB was performed to measure the BCL-XL and released cytochrome c proteins from mitochondria; Caspase-3/7 activity was measured using the Caspase-Glo 3/7 reagent from the ApoTox-Glo Triplex Assay kit induce Positive 299
Cisplatin 24 hr Ishikawa and Caco-2 WB was performed to measure the BCL-XL and released cytochrome c proteins from mitochondria; Caspase-3/7 activity was measured using the Caspase-Glo 3/7 reagent from the ApoTox-Glo Triplex Assay kit induce Positive 299
Cadmium 100, 500 μM 30 min rat PC12 cells measured spectrofluorometrically using the fluorescent dye dichlorodihydrofluorescein diacetate (DCFH2-DA) as the ROS-sensitive probe induce Positive 300
Mercury 10-500 μM 30min rat PC12 cells measured spectrofluorometrically using the fluorescent dye dichlorodihydrofluorescein diacetate (DCFH2-DA) as the ROS-sensitive probe induce Positive 300
Copper 10-500 μM 3hr rat PC12 cells measured spectrofluorometrically using the fluorescent dye dichlorodihydrofluorescein diacetate (DCFH2-DA) as the ROS-sensitive probe induce Positive 300
Copper 500 μM 24 hr rat PC12 cells measured spectrofluorometrically using the fluorescent dye dichlorodihydrofluorescein diacetate (DCFH2-DA) as the ROS-sensitive probe induce Positive 300