Compound	Dose	Time	Species	Model	Method	Action	Result	Positive criterion	Reference
Zidovudine						Increase	Positive		307
Acyclovir						Increase	Positive		307
Ganciclovir						Increase	Positive		307
Zalcitabine						Increase	Positive		307
amphotericin B	2.5mg/ml		Aspergillus fumigatus		oxidant-sensing fluorescent probe 2′,7′-dichlorofluorescin diacetate t	induce	Positive		304
Terbinafine	4mg/ml		Aspergillus fumigatus		oxidant-sensing fluorescent probe 2′,7′-dichlorofluorescin diacetate t	induce	Positive		304
itraconazole	4mg/ml		Aspergillus fumigatus		oxidant-sensing fluorescent probe 2′,7′-dichlorofluorescin diacetate t	induce	Positive		304
P110	1 µM	2hr		SH-SY5Y cells were treated with P110 (1 µM) followed by incubation of MPP+ (2 mM) for 2 hours.	Mitochondrial superoxide production was determined using the mitochondrial superoxide indicator MitoSOX Red. Total ROS production was determined in SH-SY5Y cells using CM2-HDCFA.	reduce	Positive		310
Clofibrate							Positive		185
Amiodarone							Positive		185
neomycin	50 μM		zebrafish	zebrafish lateral line hair	use ROS indicator dye cellROX green ( it labels a number of intracellular compartments, including cytoplasm, nucleus, and mitochondria)	increase	Positive		315
Staurosporine	2 µM	24hr		U2OS	mt-roGFP and mitoSOX	increase	Positive		313
camptothecin	30 µM	24hr		U2OS	mt-roGFP and mitoSOX	increase	Positive		313
Menadione	20 µM	24hr		U2OS	mt-roGFP and mitoSOX	increase	Positive		313
Cisplatin	150 µM	24hr		U2OS	U2OS cells stably expressing mt-roGFP and HyPer-red targeted at mitochondria	increase	Positive		313
camptothecin	30 µM	24hr		U2OS	U2OS cells stably expressing mt-roGFP and HyPer-red targeted at mitochondria	increase	Positive		313
Ciprofloxacin						Increase	Positive		307
Mitoquinone				U2OS	mt-roGFP ratio change (Quantitative representation of the percentage of cell death in cells pre-treated with MitoQ followed by drug treatment or drug alone as indicated for 24 h)	reduce	Positive		313
Cisplatin						Increase	Positive		307
Cyclophosphamide						Increase	Positive		307
Gentamicin	5mM	10min	rat	isolated rat liver (and kidney) mitochondria	Mitochondrial ROS production can be measured using Amplex Red, a dye that fluoresces dependent on hydrogen peroxide (H2O2) levels.	reduce	Positive		316
glyceraldehyde 3-phosphate	2.5mM					decrease	Positive		162
dihydroxyacetone phosphate						decrease	Positive		162
Berberine	25 μM	6, 24 hr		K1735-M2 mouse melanoma cells	CM-H2DCFDA (DCF), a fluorescent probe for oxidative stress (epifluorescence microscope)	increase	Positive		327
Acetaminophen						increase	Positive		35
Menadione						increase	Positive		35
Troglitazone			human	hepatocytes	cell viability assay		Positive		226
CGP37157	1 μmol/L		rat	ventricular myocytes	redox-sensitive OMM-HyPer and ERroGFP_iE biosensors		Positive		241
kaempferol	10 μmol/L		rat	ventricular myocytes	redox-sensitive OMM-HyPer and ERroGFP_iE biosensors		Positive		241
MitoTEMPO	20 μmol/L						Positive		241
Amiodarone	1 μmol/L	1 hour	human	HepG2	Measurement of ROS	increase	Positive	p < 0.05	4
Amiodarone	100 μmol/L	1 hour	human	HepG2	Measurement of ROS	increase	Positive	p < 0.05	4
benzarone	10 μmol/L	1 hour	human	HepG2	Measurement of ROS	increase	Positive	started to be detectable	4
benzarone	100 μmol/L	1 hour	human	HepG2	Measurement of ROS	increase	Positive	p < 0.01	4
benzbromarone	0.1 nmol/L	1 hour	human	HepG2	Measurement of ROS	increase	Positive	started to be detectable	4
benzbromarone	0.1 μmol/L	1 hour	human	HepG2	Measurement of ROS	increase	Positive	p < 0.05	4
benzbromarone	1 μmol/L	1 hour	human	HepG2	Measurement of ROS	increase	Positive	p < 0.01	4
benzbromarone	100 μmol/L	1 hour	human	HepG2	Measurement of ROS	increase	Positive	p < 0.01	4
Sulindac						increase	Positive		45
Diflunisal	558		rat	hepatocytes	use CM-H2DCFDA to monitor reactive oxygen species		Positive	AC50 (μM)	40
Sulindac sulfide	109		rat	hepatocytes	use CM-H2DCFDA to monitor reactive oxygen species		Positive	AC50 (μM)	40
Nimesulide	574		rat	hepatocytes	use CM-H2DCFDA to monitor reactive oxygen species		Positive	AC50 (μM)	40
Acetaminophen							Positive		243
Acetylcysteine					rho-GFP (Redox-sensitive GFP)		Positive		244
Amitriptyline							Positive		197
Desipramine							Positive		197
Valproic Acid						increase	Positive		197
Doxorubicin						increase	Positive		197
Efavirenz						increase	Positive		197
Propofol						increase	Positive		197
Ketamine						increase	Positive		197
Paraquat	10 mg/kg/week intraperitoneal (i.p.)	4 weeks	C57BL/6 mice				Positive		242
Paraquat	250 μM	5min	Sprague Dawley rat	brain cultures			Positive		242
Rotenone	0.1–0.5 nmol/mg protein		Wistar rat	brain isolated mitochondria		increase	Positive		242
Manganese	0.1–10 μM			Sprague Dawley rat astrocytes and microglia cell culture		reduce	Positive		242
Manganese	15 mg/l in drinking water		Swiss Albino mice			increase	Positive		242
Aluminium	10 mg/kg/day intragastric for 12 weeks		Wistar rats				Positive		242
Methylmercury	15 mg/l in drinking water		Swiss albino mice				Positive		242
Ethanol			rat	liver			Positive		259
Acetaminophen							Positive		258
Doxorubicin							Positive		250
Etomoxir	5 μM			T cell			Positive		257
Ubidecarenone							Positive		248
Mitoquinone							Positive		248
Acetaminophen	300 mg/kg	3 hours	mice; C57Bl/6	in vivo	ALT activity measurement	increase	Positive	p < 0.05	1
Acetaminophen	300 mg/kg	6 hours	mice; C57Bl/6	in vivo	ALT activity measurement	increase	Positive	p < 0.05	1
Cadmium	50, 100 μM			HPNE cell (human pancreatic Nestin-expressing cells)	oxidative stress was assessed by measuring DCF fluorescence	increase	Positive		334
Cadmium	50, 100 μM			AsPC-1 cells (pancreatic tumor cells)	oxidative stress was assessed by measuring DCF fluorescence	increase	Positive		334
Acetaminophen	1.5 g/kg	24 hours	rat; Fischer (F344)	in vivo	ALT activity measurement	increase	Positive	p < 0.05	1
Acetaminophen	300 mg/kg	24 hours	mice; C57Bl/6	in vivo	ALT activity measurement	increase	Positive	p < 0.05	1
Acetylsalicylic acid	10 μmol/ml	24 hours	human	HepG2	ROS measurement	increase	Positive	p < 0.05	2
Acetylsalicylic acid	10 μmol/ml	48 hours	human	HepG2	ROS measurement	increase	Positive	p < 0.05	2
Nimesulide	1 mM		rat; Sprague-Dawley Crl:CD (SD) BR	lung microsomes	adrenochrome assay (Misra and Fridovich, 1972)	increase	Positive	p < 0.05	11
Nimesulide	0.5 mM	20 minutes	rat; Sprague-Dawley Crl:CD (SD) BR	lung microsomes	horseadish preoxidase method (Green and Hill, 1984)	increase	Positive	p < 0.05	11
Rotenone	100 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
tacrolimus	50 µg/ml	12hr		INS-1 cells	MitoSOX Red staining to detect mitochondrial superoxide anion (O2−) levels by flow cytometric analysis and e time-lapse images of cells treated for 12 h with MitoSOX Red.	induce	Positive		322
Paraquat	10 mM		yeast; EG110 (MnSOD knock-out)	mitochondria	Measurement of superoxide anion; Used ethanol (5 mM) as substrate	increase	Positive		14
Paraquat	10 mM		yeast; EG103 (Wild-type)	mitochondria	Measurement of superoxide anion; Used ethanol (5 mM) as substrate	increase	Positive		14
Paraquat	1 mM	10 minutes	rat	isolated heart mitochondria	Measurement of superoxide anion; Used glutamate/malate (5 mM) as substrate	increase	Positive		14
Paraquat	0.1 mM	10 minutes	rat	isolated heart mitochondria	Measurement of superoxide anion; Used succinate (5 mM) as substrate	increase	Positive		14
Paraquat	1 mM	10 minutes	rat	isolated heart mitochondria	Measurement of hydrogen peroxide; Used glutamate/malate (5 mM) as substrate	increase	Positive		14
Paraquat	1 mM	10 minutes	rat	isolated heart mitochondria	Measurement of hydrogen peroxide; Used succinate (5 mM) as substrate	increase	Positive		14
MG-132	20 μM	24 hr	rat	PC12 cells	Measurement of intracellular ROS formation with DCFH2-DA	increase	Positive		289
3-morpholinosydnonimine	150 μM	24 hr	rat	PC12 cells	Measurement of intracellular ROS formation with DCFH2-DA	decrease	Positive		289
Cisplatin	84.96 ± 3, 158.9 ± 8.2, 372.7 ± 17.5 and 499.5 ± 15.1 μM	24 hr		Ishikawa, MDA-MB-231, PC-3 and Caco-2	Intracellular ROS (a) and mitochondrial superoxide were measured by fluorescence intensity of H2DCFDA and MitoSOX respectively.	increase	Positive	IC50	299
Dequalinium Chloride	14.24 ± 0.59, 57.85 ± 3.59, 93.31 ± 3.21 and 179.2 ± 5.2 μM	24 hr		Ishikawa, MDA-MB-231, PC-3 and Caco-2	Intracellular ROS (a) and mitochondrial superoxide were measured by fluorescence intensity of H2DCFDA and MitoSOX respectively.	increase	Positive	IC50	299
Metformin	1 mM	24 hours	human	hepatocytes	ROS measurement	increase	Positive		15
Metformin	2 mM	24 hours	human	hepatocytes	ROS measurement	increase	Positive		15
buformin	75 μM	24 hours	human	hepatocytes	ROS measurement	increase	Positive		15
Phenformin	12.5 μM	24 hours	human	hepatocytes	ROS measurement	increase	Positive		15
Amiodarone	10 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
Chlorpromazine	50 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
Cyclosporin A	5 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
imipramine	50 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
Maprotiline	5 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
Metformin	1 mM	24 hours	human	hepatocytes	Measurement of mitochondrial membrane potential	affect	Positive		15
Metformin	2 mM	24 hours	human	hepatocytes	Measurement of mitochondrial membrane potential	affect	Positive		15
buformin	75 μM	24 hours	human	hepatocytes	Measurement of mitochondrial membrane potential	affect	Positive		15
Phenformin	12.5 μM	24 hours	human	hepatocytes	Measurement of mitochondrial membrane potential	affect	Positive		15
Tamoxifen	5 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
Troglitazone	10 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
atorvastatin	100 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
Cerivastatin	5 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
fluvastatin	50 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
lovastatin	10 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
pravastatin	50 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
Simvastatin	5 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306

F0501. Heart-specific oxidative stress
F0502. Liver-specific oxidative stress
F0503. Neuron-specific oxidative stress
F0504. ROS production
F0505. ROS detoxification
F0506. RSS production
F050401. H2O2 production
F050501. antioxidant production
F050502. scavenger of mitochondrial superoxide

T001. NADH:ubiquinone reductase
T004. Quinol--cytochrome-c reductase
T021001. Carnitine O-palmitoyltransferase 1, liver isoform, CPT1-L, EC 2.3.1.21
T181. Glycerol-3-phosphate dehydrogenase, mitochondrial
T358. Dynamin-1-like protein
T387. Reactive oxygen species
T387001. superoxide
T387002. hydrogen peroxide
T400. oxidized glutathione