F05 | Oxidative stress


Oxidative stress

Compound Dose Time Species Model Method Action Result Positive criterion Reference
Zidovudine Increase Positive 307
Acyclovir Increase Positive 307
Ganciclovir Increase Positive 307
Zalcitabine Increase Positive 307
amphotericin B 2.5mg/ml Aspergillus fumigatus oxidant-sensing fluorescent probe 2′,7′-dichlorofluorescin diacetate t induce Positive 304
Terbinafine 4mg/ml Aspergillus fumigatus oxidant-sensing fluorescent probe 2′,7′-dichlorofluorescin diacetate t induce Positive 304
itraconazole 4mg/ml Aspergillus fumigatus oxidant-sensing fluorescent probe 2′,7′-dichlorofluorescin diacetate t induce Positive 304
P110 1 µM 2hr SH-SY5Y cells were treated with P110 (1 µM) followed by incubation of MPP+ (2 mM) for 2 hours. Mitochondrial superoxide production was determined using the mitochondrial superoxide indicator MitoSOX Red. Total ROS production was determined in SH-SY5Y cells using CM2-HDCFA. reduce Positive 310
Clofibrate Positive 185
Amiodarone Positive 185
neomycin 50 μM zebrafish zebrafish lateral line hair use ROS indicator dye cellROX green ( it labels a number of intracellular compartments, including cytoplasm, nucleus, and mitochondria) increase Positive 315
Staurosporine 2 µM 24hr U2OS mt-roGFP and mitoSOX increase Positive 313
camptothecin 30 µM 24hr U2OS mt-roGFP and mitoSOX increase Positive 313
Menadione 20 µM 24hr U2OS mt-roGFP and mitoSOX increase Positive 313
Cisplatin 150 µM 24hr U2OS U2OS cells stably expressing mt-roGFP and HyPer-red targeted at mitochondria increase Positive 313
camptothecin 30 µM 24hr U2OS U2OS cells stably expressing mt-roGFP and HyPer-red targeted at mitochondria increase Positive 313
Ciprofloxacin Increase Positive 307
Mitoquinone U2OS mt-roGFP ratio change (Quantitative representation of the percentage of cell death in cells pre-treated with MitoQ followed by drug treatment or drug alone as indicated for 24 h) reduce Positive 313
Cisplatin Increase Positive 307
Cyclophosphamide Increase Positive 307
Gentamicin 5mM 10min rat isolated rat liver (and kidney) mitochondria Mitochondrial ROS production can be measured using Amplex Red, a dye that fluoresces dependent on hydrogen peroxide (H2O2) levels. reduce Positive 316
glyceraldehyde 3-phosphate 2.5mM decrease Positive 162
dihydroxyacetone phosphate decrease Positive 162
Berberine 25 μM 6, 24 hr K1735-M2 mouse melanoma cells CM-H2DCFDA (DCF), a fluorescent probe for oxidative stress (epifluorescence microscope) increase Positive 327
Acetaminophen increase Positive 35
Menadione increase Positive 35
Troglitazone human hepatocytes cell viability assay Positive 226
CGP37157 1 μmol/L rat ventricular myocytes redox-sensitive OMM-HyPer and ERroGFP_iE biosensors Positive 241
kaempferol 10 μmol/L rat ventricular myocytes redox-sensitive OMM-HyPer and ERroGFP_iE biosensors Positive 241
MitoTEMPO 20 μmol/L Positive 241
Amiodarone 1 μmol/L 1 hour human HepG2 Measurement of ROS increase Positive p < 0.05 4
Amiodarone 100 μmol/L 1 hour human HepG2 Measurement of ROS increase Positive p < 0.05 4
benzarone 10 μmol/L 1 hour human HepG2 Measurement of ROS increase Positive started to be detectable 4
benzarone 100 μmol/L 1 hour human HepG2 Measurement of ROS increase Positive p < 0.01 4
benzbromarone 0.1 nmol/L 1 hour human HepG2 Measurement of ROS increase Positive started to be detectable 4
benzbromarone 0.1 μmol/L 1 hour human HepG2 Measurement of ROS increase Positive p < 0.05 4
benzbromarone 1 μmol/L 1 hour human HepG2 Measurement of ROS increase Positive p < 0.01 4
benzbromarone 100 μmol/L 1 hour human HepG2 Measurement of ROS increase Positive p < 0.01 4
Sulindac increase Positive 45
Diflunisal 558 rat hepatocytes use CM-H2DCFDA to monitor reactive oxygen species Positive AC50 (μM) 40
Sulindac sulfide 109 rat hepatocytes use CM-H2DCFDA to monitor reactive oxygen species Positive AC50 (μM) 40
Nimesulide 574 rat hepatocytes use CM-H2DCFDA to monitor reactive oxygen species Positive AC50 (μM) 40
Acetaminophen Positive 243
Acetylcysteine rho-GFP (Redox-sensitive GFP) Positive 244
Amitriptyline Positive 197
Desipramine Positive 197
Valproic Acid increase Positive 197
Doxorubicin increase Positive 197
Efavirenz increase Positive 197
Propofol increase Positive 197
Ketamine increase Positive 197
Paraquat 10 mg/kg/week intraperitoneal (i.p.) 4 weeks C57BL/6 mice Positive 242
Paraquat 250 μM 5min Sprague Dawley rat brain cultures Positive 242
Rotenone 0.1–0.5 nmol/mg protein Wistar rat brain isolated mitochondria increase Positive 242
Manganese 0.1–10 μM Sprague Dawley rat astrocytes and microglia cell culture reduce Positive 242
Manganese 15 mg/l in drinking water Swiss Albino mice increase Positive 242
Aluminium 10 mg/kg/day intragastric for 12 weeks Wistar rats Positive 242
Methylmercury 15 mg/l in drinking water Swiss albino mice Positive 242
Ethanol rat liver Positive 259
Acetaminophen Positive 258
Doxorubicin Positive 250
Etomoxir 5 μM T cell Positive 257
Ubidecarenone Positive 248
Mitoquinone Positive 248
Acetaminophen 300 mg/kg 3 hours mice; C57Bl/6 in vivo ALT activity measurement increase Positive p < 0.05 1
Acetaminophen 300 mg/kg 6 hours mice; C57Bl/6 in vivo ALT activity measurement increase Positive p < 0.05 1
Cadmium 50, 100 μM HPNE cell (human pancreatic Nestin-expressing cells) oxidative stress was assessed by measuring DCF fluorescence increase Positive 334
Cadmium 50, 100 μM AsPC-1 cells (pancreatic tumor cells) oxidative stress was assessed by measuring DCF fluorescence increase Positive 334
Acetaminophen 1.5 g/kg 24 hours rat; Fischer (F344) in vivo ALT activity measurement increase Positive p < 0.05 1
Acetaminophen 300 mg/kg 24 hours mice; C57Bl/6 in vivo ALT activity measurement increase Positive p < 0.05 1
Acetylsalicylic acid 10 μmol/ml 24 hours human HepG2 ROS measurement increase Positive p < 0.05 2
Acetylsalicylic acid 10 μmol/ml 48 hours human HepG2 ROS measurement increase Positive p < 0.05 2
Nimesulide 1 mM rat; Sprague-Dawley Crl:CD (SD) BR lung microsomes adrenochrome assay (Misra and Fridovich, 1972) increase Positive p < 0.05 11
Nimesulide 0.5 mM 20 minutes rat; Sprague-Dawley Crl:CD (SD) BR lung microsomes horseadish preoxidase method (Green and Hill, 1984) increase Positive p < 0.05 11
Rotenone 100 µM 1 hour Human HepG2 High-content screening assay Increase Positive MEC 306
tacrolimus 50 µg/ml 12hr INS-1 cells MitoSOX Red staining to detect mitochondrial superoxide anion (O2−) levels by flow cytometric analysis and e time-lapse images of cells treated for 12 h with MitoSOX Red. induce Positive 322
Paraquat 10 mM yeast; EG110 (MnSOD knock-out) mitochondria Measurement of superoxide anion; Used ethanol (5 mM) as substrate increase Positive 14
Paraquat 10 mM yeast; EG103 (Wild-type) mitochondria Measurement of superoxide anion; Used ethanol (5 mM) as substrate increase Positive 14
Paraquat 1 mM 10 minutes rat isolated heart mitochondria Measurement of superoxide anion; Used glutamate/malate (5 mM) as substrate increase Positive 14
Paraquat 0.1 mM 10 minutes rat isolated heart mitochondria Measurement of superoxide anion; Used succinate (5 mM) as substrate increase Positive 14
Paraquat 1 mM 10 minutes rat isolated heart mitochondria Measurement of hydrogen peroxide; Used glutamate/malate (5 mM) as substrate increase Positive 14
Paraquat 1 mM 10 minutes rat isolated heart mitochondria Measurement of hydrogen peroxide; Used succinate (5 mM) as substrate increase Positive 14
MG-132 20 μM 24 hr rat PC12 cells Measurement of intracellular ROS formation with DCFH2-DA increase Positive 289
3-morpholinosydnonimine 150 μM 24 hr rat PC12 cells Measurement of intracellular ROS formation with DCFH2-DA decrease Positive 289
Cisplatin 84.96 ± 3, 158.9 ± 8.2, 372.7 ± 17.5 and 499.5 ± 15.1 μM 24 hr Ishikawa, MDA-MB-231, PC-3 and Caco-2 Intracellular ROS (a) and mitochondrial superoxide were measured by fluorescence intensity of H2DCFDA and MitoSOX respectively. increase Positive IC50 299
Dequalinium Chloride 14.24 ± 0.59, 57.85 ± 3.59, 93.31 ± 3.21 and 179.2 ± 5.2 μM 24 hr Ishikawa, MDA-MB-231, PC-3 and Caco-2 Intracellular ROS (a) and mitochondrial superoxide were measured by fluorescence intensity of H2DCFDA and MitoSOX respectively. increase Positive IC50 299
Metformin 1 mM 24 hours human hepatocytes ROS measurement increase Positive 15
Metformin 2 mM 24 hours human hepatocytes ROS measurement increase Positive 15
buformin 75 μM 24 hours human hepatocytes ROS measurement increase Positive 15
Phenformin 12.5 μM 24 hours human hepatocytes ROS measurement increase Positive 15
Amiodarone 10 µM 1 hour Human HepG2 High-content screening assay Increase Positive MEC 306
Chlorpromazine 50 µM 1 hour Human HepG2 High-content screening assay Increase Positive MEC 306
Cyclosporin A 5 µM 1 hour Human HepG2 High-content screening assay Increase Positive MEC 306
imipramine 50 µM 1 hour Human HepG2 High-content screening assay Increase Positive MEC 306
Maprotiline 5 µM 1 hour Human HepG2 High-content screening assay Increase Positive MEC 306
Metformin 1 mM 24 hours human hepatocytes Measurement of mitochondrial membrane potential affect Positive 15
Metformin 2 mM 24 hours human hepatocytes Measurement of mitochondrial membrane potential affect Positive 15
buformin 75 μM 24 hours human hepatocytes Measurement of mitochondrial membrane potential affect Positive 15
Phenformin 12.5 μM 24 hours human hepatocytes Measurement of mitochondrial membrane potential affect Positive 15
Tamoxifen 5 µM 1 hour Human HepG2 High-content screening assay Increase Positive MEC 306
Troglitazone 10 µM 1 hour Human HepG2 High-content screening assay Increase Positive MEC 306
atorvastatin 100 µM 1 hour Human HepG2 High-content screening assay Increase Positive MEC 306
Cerivastatin 5 µM 1 hour Human HepG2 High-content screening assay Increase Positive MEC 306
fluvastatin 50 µM 1 hour Human HepG2 High-content screening assay Increase Positive MEC 306
lovastatin 10 µM 1 hour Human HepG2 High-content screening assay Increase Positive MEC 306
pravastatin 50 µM 1 hour Human HepG2 High-content screening assay Increase Positive MEC 306
Simvastatin 5 µM 1 hour Human HepG2 High-content screening assay Increase Positive MEC 306